High-resolution plasma metabolomics and thiamine status in critically Ill adult patients.

Autor: Gundogan K; Division of Intensive Care Medicine, Department of Internal Medicine, Erciyes University School of Medicine, Melikgazi, 38039, Kayseri, Turkey. kgundogan@erciyes.edu.tr.; Division of Clinical Nutrition, Erciyes University Health Sciences Institute, Kayseri, Turkey. kgundogan@erciyes.edu.tr., Nellis MM; Clinical Biomarkers Laboratory, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Ozer NT; Division of Clinical Nutrition, Erciyes University Health Sciences Institute, Kayseri, Turkey., Ergul SS; Division of Clinical Nutrition, Erciyes University Health Sciences Institute, Kayseri, Turkey., Sahin GG; Division of Clinical Nutrition, Erciyes University Health Sciences Institute, Kayseri, Turkey., Temel S; Division of Intensive Care Medicine, Department of Internal Medicine, Erciyes University School of Medicine, Melikgazi, 38039, Kayseri, Turkey., Yuksel RC; Division of Intensive Care Medicine, Department of Internal Medicine, Erciyes University School of Medicine, Melikgazi, 38039, Kayseri, Turkey., Teeny S; Clinical Biomarkers Laboratory, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Alvarez JA; Division of Endocrinology, Metabolism, and Lipids, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA.; Department of Medicine, Emory Center for Clinical and Molecular Nutrition, Emory University School of Medicine, Atlanta, GA, USA., Sungur M; Division of Intensive Care Medicine, Department of Internal Medicine, Erciyes University School of Medicine, Melikgazi, 38039, Kayseri, Turkey.; Division of Clinical Nutrition, Erciyes University Health Sciences Institute, Kayseri, Turkey., Jones DP; Clinical Biomarkers Laboratory, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Department of Medicine, Emory Center for Clinical and Molecular Nutrition, Emory University School of Medicine, Atlanta, GA, USA.; Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA., Ziegler TR; Division of Endocrinology, Metabolism, and Lipids, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA.; Department of Medicine, Emory Center for Clinical and Molecular Nutrition, Emory University School of Medicine, Atlanta, GA, USA.
Jazyk: angličtina
Zdroj: Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2024 Jul 27; Vol. 20 (4), pp. 83. Date of Electronic Publication: 2024 Jul 27.
DOI: 10.1007/s11306-024-02144-9
Abstrakt: Introduction: Thiamine (Vitamin B1) is an essential micronutrient and is classically considered a co-factor in energy metabolism. The association between thiamine status and whole-body metabolism in critical illness has not been studied.
Objectives: To determine association between whole blood thiamine pyrophosphate (TPP) concentrations and plasma metabolites and connected metabolic pathways using high resolution metabolomics (HRM) in critically ill patients.
Methods: Cross-sectional study performed at Erciyes University Hospital, Kayseri, Turkey and Emory University, Atlanta, GA, USA. Participants were critically ill adults with an expected length of intensive care unit stay longer than 48 h and receiving chronic furosemide therapy. A total of 76 participants were included. Mean age was 69 years (range 33-92 years); 65% were female. Blood for TPP and metabolomics was obtained on the day of ICU admission. Whole blood TPP was measured by HPLC and plasma HRM was performed using liquid chromatography/mass spectrometry. Data was analyzed using regression analysis of TPP levels against all plasma metabolomic features in metabolome-wide association studies (MWAS). MWAS using the highest and lowest TPP concentration tertiles was performed as a secondary analysis.
Results: Specific metabolic pathways associated with whole blood TPP levels in regression and tertile analysis included pentose phosphate, fructose and mannose, branched chain amino acid, arginine and proline, linoleate, and butanoate pathways.
Conclusions: Plasma HRM revealed that thiamine status, determined by whole blood TPP concentrations, was significantly associated with metabolites and metabolic pathways related to metabolism of energy, carbohydrates, amino acids, lipids, and the gut microbiome in adult critically ill patients.
(© 2024. The Author(s).)
Databáze: MEDLINE
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