| Autor: |
Issac Abraham SVP; Division of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641 114, Tamil Nadu, India., Arumugam VR; Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India., Mary NI; Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India., Dharmadhas JS; Department of Biochemistry and Biotechnology, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore 641 043, Tamil Nadu, India., Sundararaj R; Centre for Drug Discovery, Karpagam Academy of Higher Education, Coimbatore 641 021, Tamil Nadu, India., Devanesan AA; Department of Biotechnology, The American College, Satellite Campus, Madurai 625 503, Tamil Nadu, India., Rajamanickam R; Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), Tiruchirappalli 620 005, Tamil Nadu, India., Veerapandian R; Department of Molecular and Translational Medicine, Center of Emphasis in Infectious Diseases, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA., John Bosco JP; Division of Electronics and Communication Engineering, Karunya Institute of Technology and Sciences, Coimbatore 641 114, Tamil Nadu, India., Danaraj J; Centre for Ocean Research, Sathyabama Research Park, Sathyabama Institute of Science and Technology, Chennai 600 119, Tamil Nadu, India. |
| Abstrakt: |
Biofilms play a decisive role in the infectious process and the development of antibiotic resistance. The establishment of bacterial biofilms is regulated by a signal-mediated cell-cell communication process called "quorum sensing" (QS). The identification of quorum sensing inhibitors (QSI) to mitigate the QS process may facilitate the development of novel treatment strategies for biofilm-based infections. In this study, the traditional medicinal plant Ocimum sanctum was screened for QS inhibitory potential. Sub-MICs of the extract significantly affected the secretion of EPS in Gram-negative human pathogens such as Escherichia coli , Pseudomonas aeruginosa PAO1, Proteus mirabilis , and Serratia marcescens , as well as aquaculture pathogens Vibrio harveyi , V. parahaemolyticus , and V. vulnificus , which render the bacteria more sensitive, leading to a loss of bacterial biomass from the substratum. The observed inhibitory activity of the O. sanctum extract might be attributed to the presence of eugenol, as evidenced through ultraviolet (UV)-visible, gas chromatography-mass spectroscopy (GC-MS), Fourier transformer infrared (FTIR) spectroscopy analyses, and computational studies. Additionally, the QSI potential of eugenol was corroborated through in vitro studies using the marker strain Chromobacterium violaceum . |
