| Autor: |
Sliskovic L; Department for Forensic Sciences, University of Split, 21 000 Split, Croatia., Milos I; Department of Integrative Physiology, School of Medicine, University of Split, 21 000 Split, Croatia., Zecic A; Department for Forensic Sciences, University of Split, 21 000 Split, Croatia., Kuret S; Department of Health Studies, University of Split, 21 000 Split, Croatia., Sutlovic D; Department of Health Studies, University of Split, 21 000 Split, Croatia.; Department of Toxicology and Pharmacogenetics, School of Medicine, University of Split, 21 000 Split, Croatia. |
| Jazyk: |
angličtina |
| Zdroj: |
Genes [Genes (Basel)] 2024 Jul 06; Vol. 15 (7). Date of Electronic Publication: 2024 Jul 06. |
| DOI: |
10.3390/genes15070888 |
| Abstrakt: |
The aim of this study was to investigate the effect of sunlight on the degradation of DNA samples taken from blood stains from different types of surfaces. A blood sample obtained from a single male donor was placed on seven different surfaces (galvanized sheet, iron rod, newspaper, white printer paper, glass, soil, and ceramic panel). Samples were kept, during a 4-week summer period, in a room, but next to an open window. Every 7 days, 1 mm 2 of blood sample was collected from each substrate and stored in labeled tube for later analysis. DNA was extracted with the Chelex method, amplified using AmpFISTR TM Minifiler TM Plus Amplification Kit, and quantified using a Quantifiler TM Human DNA Quantification kit. After 7 days of sun exposure, the highest DNA concentration was determined to be from the sample from a galvanized sheet stain, followed by, in order of decreasing concentration, the ceramic panel, glass, newspaper, iron rod, and white printer paper surface. As expected, the DNA concentration from all samples decreased as the sunlight exposure time progressed. The results obtained after the amplification in the MiniFiler TM system were in correlation with the DNA concentrations measured by the qPCR method for all samples, except for the glass, soil, and white printer paper samples. The obtained data show that DNA degradation is correlated to the length of sunlight exposure and to the type of surface the samples are collected from. A negative qPCR result does not mean negative PCR amplification in the STR system; therefore, both methods should be applied when analyzing forensic samples collected from trace evidence. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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