| Autor: |
Mejía-Méndez JL; Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula 72810, Puebla, Mexico., Sánchez-Ante G; Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Gral. Ramón Corona No 2514, Colonia Nuevo México, Zapopan 45121, Jalisco, Mexico., Cerro-López M; Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula 72810, Puebla, Mexico., Minutti-Calva Y; Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula 72810, Puebla, Mexico., Navarro-López DE; Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Gral. Ramón Corona No 2514, Colonia Nuevo México, Zapopan 45121, Jalisco, Mexico., Lozada-Ramírez JD; Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula 72810, Puebla, Mexico., Bach H; Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, BC V6H 3Z6, Canada., López-Mena ER; Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Gral. Ramón Corona No 2514, Colonia Nuevo México, Zapopan 45121, Jalisco, Mexico., Sánchez-Arreola E; Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula 72810, Puebla, Mexico. |
| Abstrakt: |
In this work, the hexane, chloroform, and methanol extracts from Kalanchoe fedtschenkoi were utilized to green-synthesize silver nanoparticles (Kf 1 -, Kf 2 -, and Kf 3 -AgNPs). The Kf 1 -, Kf 2 -, and Kf 3 -AgNPs were characterized by spectroscopy and microscopy techniques. The antibacterial activity of AgNPs was studied against bacteria strains, utilizing the microdilution assay. The DPPH and H 2 O 2 assays were considered to assess the antioxidant activity of AgNPs. The results revealed that Kf 1 -, Kf 2 -, and Kf 3 -AgNPs exhibit an average diameter of 39.9, 111, and 42 nm, respectively. The calculated ζ-potential of Kf 1 -, Kf 2 -, and Kf 3 -AgNPs were -20.5, -10.6, and -7.9 mV, respectively. The UV-vis analysis of the three samples demonstrated characteristic absorption bands within the range of 350-450 nm, which confirmed the formation of AgNPs. The FTIR analysis of AgNPs exhibited a series of bands from 3500 to 750 cm -1 , related to the presence of extracts on their surfaces. SEM observations unveiled that Kf 1 - and Kf 2 -AgNPs adopted structural arrangements related to nano-popcorns and nanoflowers, whereas Kf 3 -AgNPs were spherical in shape. It was determined that treatment with Kf 1 -, Kf 2 -, and Kf 3 -AgNPs was demonstrated to inhibit the growth of E. coli , S. aureus , and P. aeruginosa in a dose-dependent manner (50-300 μg/mL). Within the same range, treatment with Kf 1 -, Kf 2 -, and Kf 3 -AgNPs decreased the generation of DPPH (IC 50 57.02-2.09 μg/mL) and H 2 O 2 (IC 50 3.15-3.45 μg/mL) radicals. This study highlights the importance of using inorganic nanomaterials to improve the biological performance of plant extracts as an efficient nanotechnological approach. |
