| Autor: |
Manojlović-Stojanoski M; Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Borković-Mitić S; Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Nestorović N; Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Ristić N; Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Stefanović R; Department of Pathology and Medical Citology, University Clinical Center of Serbia, Pasterova 2, 11000 Belgrade, Serbia.; Faculty of Medicine, University of Belgrade, dr Koste Todorovića 26, 11000 Belgrde, Serbia., Stevanović M; Group for Biomedical Engineering and Nanobiotechnology, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts (SASA), Kneza Mihaila 35/IV, 11000 Belgrade, Serbia., Filipović N; Group for Biomedical Engineering and Nanobiotechnology, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts (SASA), Kneza Mihaila 35/IV, 11000 Belgrade, Serbia., Stojsavljević A; Innovative Centre, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia., Pavlović S; Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia. |
| Abstrakt: |
To compare the effects of organic selenium nanoparticles (SeNPs, Se 0 ) and inorganic sodium selenite (NaSe, Na 2 SeO 3 , Se 4+ ) on the antioxidant response in maternal and fetal rat liver, pregnant females were treated with two forms of selenium (Se) at equivalent doses during gestation (0.5 mg SeNPs or 0.5 mg NaSe/kg body weight/day). Structural parameters of the liver of gravid females and their fetuses were examined in a sex-specific manner. The oxidative stress parameters superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (GSH) and sulfhydryl groups (SH) were established. In addition, the Se concentration was determined in the blood, liver, urine and feces of the gravid females and in the liver of the fetuses. The structure of the liver of gravid females remained histologically the same after supplementation with both forms of Se, while the oxidative stress in the liver was significantly lower after the use of SeNPs compared to NaSe. Immaturity of fetal antioxidant defenses and sex specificity were demonstrated. This study provides a detailed insight into the differences in the bioavailability of the nano form of Se compared to sodium selenite in the livers of pregnant females and fetuses. |
