| Autor: |
Mutavdzin Krneta S; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Gopcevic K; Institute of Chemistry in Medicine 'Prof. Dr. Petar Matavulj', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Stankovic S; Centre for Medical Biochemistry, University Clinical Centre of Serbia, 11000 Belgrade, Serbia.; Department of Biochemistry, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia., Jakovljevic Uzelac J; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Todorovic D; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Labudovic Borovic M; Institute of Histology and Embryology 'Aleksandar Dj. Kostic', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Rakocevic J; Institute of Histology and Embryology 'Aleksandar Dj. Kostic', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Djuric D; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. |
| Abstrakt: |
The aims of this study were to examine the effects of pyridoxine administration on the activities of cardiac antioxidant stress enzymes superoxide dismutase (SOD) and catalase (CAT) and enzyme indicators of cardiometabolic status, lactate and malate dehydrogenase (LDH, MDH), as well as LDH and MDH isoforms' distribution in the cardiac tissue of healthy and diabetic Wistar male rats. Experimental animals were divided into five groups: C1-control (0.9% sodium chloride-NaCl-1 mL/kg, intraperitoneally (i.p.), 1 day); C2-second control (0.9% NaCl 1 mL/kg, i.p., 28 days); DM-diabetes mellitus (streptozotocin 100 mg/kg in 0.9% NaCl, i.p., 1 day); P-pyridoxine (7 mg/kg, i.p., 28 days); and DM + P-diabetes mellitus and pyridoxine (streptozotocin 100 mg/kg, i.p., 1 day and pyridoxine 7 mg/kg, i.p., 28 days). Pyridoxine treatment reduced CAT and MDH activity in diabetic rats. In diabetic rats, the administration of pyridoxine increased LDH1 and decreased LDH4 isoform activities, as well as decreased peroxisomal MDH and increased mitochondrial MDH activities. Our findings highlight the positive effects of pyridoxine administration on the complex interplay between oxidative stress, antioxidant enzymes, and metabolic changes in diabetic cardiomyopathy. |
