Effects of photobiomodulation on different phases of in vitro osteogenesis.

Autor: de Araújo PPB; Division of Oral Pathology, Faculdade São Leopoldo Mandic, Rua José Rocha Junqueira 13, Swift, Campinas, SP, 13045-755, Brazil., Martinez EF; Division of Oral Pathology, Faculdade São Leopoldo Mandic, Rua José Rocha Junqueira 13, Swift, Campinas, SP, 13045-755, Brazil., Garcez AS; Division of Oral Pathology, Faculdade São Leopoldo Mandic, Rua José Rocha Junqueira 13, Swift, Campinas, SP, 13045-755, Brazil., de Castro Raucci LMS; School of Dentistry, University of Ribeirao Preto, Avenida Costábile Romano, 2201, Nova Ribeirânia, Ribeirão Preto, SP, 14096-900, Brazil., Soares AB; Division of Oral Pathology, Faculdade São Leopoldo Mandic, Rua José Rocha Junqueira 13, Swift, Campinas, SP, 13045-755, Brazil., de Araújo VC; Division of Oral Pathology, Faculdade São Leopoldo Mandic, Rua José Rocha Junqueira 13, Swift, Campinas, SP, 13045-755, Brazil., Teixeira LN; Division of Oral Pathology, Faculdade São Leopoldo Mandic, Rua José Rocha Junqueira 13, Swift, Campinas, SP, 13045-755, Brazil. lucas.teixeira@slmandic.edu.br.
Jazyk: angličtina
Zdroj: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2024 Aug; Vol. 23 (8), pp. 1565-1571. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1007/s43630-024-00616-4
Abstrakt: The present study aimed to evaluate the effect of photobiomodulation therapy (PBM) on different stages of osteogenesis in vitro. For this, osteoblastic-like cells (Saos-2 cell lineage) were irradiated in two different periods: during the Proliferation phase (PP; from the second to the fourth day) and during the Differentiation phase (DP; from the seventh to the ninth day). The energy density used in the study was 1.5 J/ cm 2 . The following parameters were evaluated: 1) quantification of collagen type 1 (COL 1), osteopontin (OPN), and bone morphogenetic protein 2 (BMP-2); 2) quantification of alkaline phosphatase (ALP) activity; and 3) quantification of  extracellular matrix (ECM) mineralization. Non-irradiated cultures were used as controls. The data were analyzed using the Student's t-test or one-way ANOVA, considering a significance level of 5%. The results indicated that COL 1 and BMP-2 quantification was higher in Saos-2 irradiated during the DP in relation to the control group at day 10 (p < 0.05). No differences were observed for other comparisons at this time point (p > 0.05). OPN expression was greater in PP compared with the other experimental groups at day 10 (p < 0.05). Irradiation did not affect ALP activity in Saos-2 regardless of the exposure phase and the time point evaluated (p > 0.05). At day 14, ECM mineralization was higher in Saos-2 cultures irradiated during the DP in relation to the PP (p < 0.05). In conclusion, the results suggested that the effects of PBM on osteoblastic cells may be influenced by the stage of cell differentiation.
(© 2024. The Author(s), under exclusive licence to the European Photochemistry Association, European Society for Photobiology.)
Databáze: MEDLINE
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