The cyanobacterial protein VIPP1 forms ESCRT-III-like structures on lipid bilayers.

Autor: Pan S; Weill Cornell Medicine, Department of Anesthesiology, New York, NY, USA., Gries K; Molecular Biotechnology and Systems Biology, RPTU Kaiserslautern-Landau, Kaiserslautern, Germany., Engel BD; Biozentrum, University of Basel, Basel, Switzerland., Schroda M; Molecular Biotechnology and Systems Biology, RPTU Kaiserslautern-Landau, Kaiserslautern, Germany., Haselwandter CA; Department of Physics and Astronomy, University of Southern California, Los Angeles, CA, USA.; Department of Quantitative and Computational Biology, University of Southern California, Los Angeles, CA, USA., Scheuring S; Weill Cornell Medicine, Department of Anesthesiology, New York, NY, USA. sis2019@med.cornell.edu.; Weill Cornell Medicine, Department of Physiology and Biophysics, New York, NY, USA. sis2019@med.cornell.edu.
Jazyk: angličtina
Zdroj: Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Jul 26. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1038/s41594-024-01367-7
Abstrakt: The biogenesis and maintenance of thylakoid membranes require vesicle-inducing protein in plastids 1 (VIPP1). VIPP1 is a member of the endosomal sorting complex required for transport-III (ESCRT-III) superfamily, whose members form diverse filament-based supramolecular structures that facilitate membrane deformation and fission. VIPP1 cryo-electron microscopy (EM) structures in solution revealed helical rods and baskets of stacked rings, with amphipathic membrane-binding domains in the lumen. However, how VIPP1 interacts with membranes remains largely unknown. Here, using high-speed atomic force microscopy (HS-AFM), we show that VIPP1 assembles into right-handed chiral spirals and regular polygons on supported lipid bilayers via ESCRT-III-like filament assembly and dynamics. VIPP1 filaments grow clockwise into spirals through polymerization at a ring-shaped central polymerization hub, and into polygons through clockwise polymerization at the sector peripheries. Interestingly, VIPP1 initially forms Archimedean spirals, which upon maturation transform into logarithmic spirals through lateral annealing of strands to the outermore low-curvature spiral turns.
Competing Interests: Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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