Fitness Differences Between Listeria monocytogenes Serotypes 1/2a, 4b, and 4bv-1 in Competition for Growth on Lettuce Leaf Sections.

Autor:	Gorski L; USDA, Agricultural Research Service, Western Regional Research Center, Produce Safety and Microbiology Unit, Albany, CA 94710, USA. Electronic address: lisa.gorski@usda.gov., Aviles Noriega A; USDA, Agricultural Research Service, Western Regional Research Center, Produce Safety and Microbiology Unit, Albany, CA 94710, USA.
Jazyk:	angličtina
Zdroj:	Journal of food protection [J Food Prot] 2024 Sep; Vol. 87 (9), pp. 100333. Date of Electronic Publication: 2024 Jul 24.
DOI:	10.1016/j.jfp.2024.100333
Abstrakt:	Listeria monocytogenes is a foodborne pathogen that lives in nature as a saprophyte. Two of the three most common serotypes that cause foodborne listeriosis are 1/2a and 4b. Within serotype 4b, there is a variant called 4bv-1. In the last decade, several produce-related outbreaks (linked to leafy salad, caramel apples, and stone fruit) were linked to 4bv-1 strains, specifically those of Sequence Type 382. This study assessed the fitness of ST 382 strains on lettuce leaf sections to determine if they are more fit on produce than strains of other serotypes. Strains of serotypes 1/2a, 4b, and ST 382 were inoculated as mixtures onto lettuce and incubated at 4 °C for 7 days or 25 °C for 24 h. Thirty L. monocytogenes colonies resulting from the growth on each lettuce piece were characterized for serotype by multiplex PCR, and the percentages of each serotype recovered were compared. In the individual mixtures with three strains, none of the ST 382 strains showed better fitness for growth on lettuce at either 4 °C or 25 °C. Overall, ST 382 strains showed better recovery from lettuce sections grown at 4 °C than at 25 °C. Statistical analysis of the recovery of twelve strains tested in competition experiments indicated that ST 382 strains were less fit for lettuce growth when competing against the other serotypes. The data indicate that ST 382 strains do not have a competitive fitness advantage on cut lettuce sections. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Published by Elsevier Inc.)
Databáze:	MEDLINE
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