Separation of weak acids, neutral compounds, and permanent anions using sequential elution liquid chromatography with tandem columns.

Autor: Lovejoy LK; R&D Medicine Development and Supply, Analytical Development, GSK, Collegeville, PA 19426, USA; Drexel University, Department of Chemistry, 32 South 32nd St., Disque Hall 305, Philadelphia, PA 19104, USA., Foley JP; Drexel University, Department of Chemistry, 32 South 32nd St., Disque Hall 305, Philadelphia, PA 19104, USA. Electronic address: jfoley@drexel.edu.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Aug 30; Vol. 1731, pp. 465178. Date of Electronic Publication: 2024 Jul 20.
DOI: 10.1016/j.chroma.2024.465178
Abstrakt: This paper discusses the development of an analytical method by an alternative separation approach, sequential elution liquid chromatography (SE-LC), to separate permanently charged ions (anions), weak acids, and neutral compounds using anion exchange and reversed-phase columns in tandem. SE-LC separates classes of compounds by group by employing two or more elution modes. Advantages to using SE-LC over conventional HPLC are a greater peak capacity and a reduced separation disorder. Importantly, the same HPLC as used for a conventional HPLC separation may be used to afford a successful SE-LC separation. Mobile phase selection and gradient optimization are integral for a successful SE-LC class separation of permanent anions, weak acids, and neutral compounds and will be discussed in detail in this paper. The most successful (best resolution and repeatability) SE-LC separation was achieved by applying isocratic elution at low pH to elute the weak acids, followed by an acetonitrile gradient to elute the neutral compounds, and last a sodium methanesulfonate gradient to elute the anionic compounds using a superficially porous C18 column coupled with a strong anion exchange (SAX) column. Repeatability (RSD) in the retention times and peak areas of the analytes was less than 0.25 % and 1.5 %, respectively.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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