High-efficient preparation of β-nicotinamide mononucleotides by crude enzymes cascade catalytic reaction.

Autor: Liu J; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China., Huo R; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China., Fu H; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China., Chen S; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China., Qiao X; Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China., Xu B; Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China., Zhang Z; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China., Wu J; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China., Su L; Key Laboratory of Industrial Biotechnology Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: sulingqia@jiangnan.edu.cn.
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2024 Oct; Vol. 180, pp. 110482. Date of Electronic Publication: 2024 Jul 20.
DOI: 10.1016/j.enzmictec.2024.110482
Abstrakt: β-nicotinamide mononucleotide (β-NMN) is a key precursor of nicotinamide adenine dinucleotide, and becomes attractive in the nutrition and health care fields, but its enzymatic synthesis is expensive. In this study, a six-enzyme cascade catalytic system was constructed to produce β-NMN. Using D-ribose and nicotinamide as substrates, the β-NMN yield reached 97.5 % catalyzed by purified enzymes. Then, after knocking out the genes encoding proteins that consume β-NMN in E. coli BL21(DE3), the similar β-NMN yield, 97.2 %, using the crude enzymes could be also obtained. After that, β-NMN synthesis was performed under increased substrate concentration, and 'modular' crude enzymes cascade catalytic reaction system was proposed to reduce the inhibition of polyphosphate on ribose-phosphate diphosphokinase activity, and the β-NMN yield reached 78.4 % at 10 mM D-ribose, which is 1.82 times of that in 'one-pot' reaction and represents the highest β-NMN preparation level with phosphoribosylpyrophosphate as the core reported till now.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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