Osmoregulation by choline-based deep eutectic solvent induces electroactivity in Bacillus subtilis biofilms.

Autor: Eghtesadi N; Department of Chemical and Materials Engineering, School of Engineering and Digital, Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 01000, Kazakhstan., Olaifa K; Department of Chemical and Materials Engineering, School of Engineering and Digital, Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 01000, Kazakhstan., Pham TT; Department of Biology, School of Sciences and Humanities, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 01000, Kazakhstan., Capriati V; Dipartimento di Farmacia - Scienze del Farmaco, Consorzio CINMPIS, Università degli Studi di Bari Aldo Moro, Bari 70125, Italy., Ajunwa OM; Interdisciplinary Nanoscience Center (iNANO), Faculty of Natural Sciences, Aarhus University, Aarhus, Denmark. Electronic address: obinna.ajunwa@inano.au.dk., Marsili E; Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo China, Ningbo, China. Electronic address: Enrico.Marsili@nottingham.edu.cn.
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2024 Oct; Vol. 180, pp. 110485. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1016/j.enzmictec.2024.110485
Abstrakt: Gram-positive Bacillus subtilis is a model organism for the biotechnology industry and has recently been characterized as weakly electroactive in both planktonic cultures and biofilms. Increasing the extracellular electron transfer (EET) rate in B. subtilis biofilms will help to develop an efficient microbial electrochemical technology (MET) and improve the bioproduction of high-value metabolites under electrofermentative conditions. In our previous work, we have shown that the addition of compatible solute precursors such as choline chloride (ChCl) to the growth medium formulation increases current output and biofilm formation in B. subtilis. In this work, we utilized a low-carbon tryptone yeast extract medium with added salts to further expose B. subtilis to salt stress and observe the osmoregulatory and/or nutritional effects of a D-sorbitol/choline chloride (ChCl) (1:1 mol mol -1 ) deep eutectic solvents (DESs) on the electroactivity of the formed biofilm. The results show that ChCl and D-sorbitol alleviate the osmotic stress induced by the addition of NaH 2 PO 4 and KH 2 PO 4 salts and boost biofilm production. This is probably due to the osmoprotective effect of ChCl, a precursor of the osmoprotectant glycine betaine, and the induction of electroactive exopolymeric substances within the B. subtilis biofilm. Since high ionic strength media are commonly used in microbial biotechnology, the combination of ChCl-containing DESs and salt stress could enhance biofilm-based electrofermentation processes that bring significant benefits for biotechnological applications.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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