Hepatic transcript profiling in beef cattle: Effects of feeding endophyte-infected tall fescue seeds.

Autor: Alfaro GF; Department of Animal Sciences, Auburn University, Auburn, AL, United States of America., Palombo V; Department of Agricultural, Environmental and Food Sciences, Università degli Studi del Molise, Campobasso, Italy., D'Andrea M; Department of Agricultural, Environmental and Food Sciences, Università degli Studi del Molise, Campobasso, Italy., Cao W; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States of America., Zhang Y; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States of America., Beever JE; Department of Animal Sciences, University of Tennessee, Knoxville, TN, United States of America., Muntifering RB; Department of Animal Sciences, Auburn University, Auburn, AL, United States of America., Pacheco WJ; Department of Poultry Sciences, Auburn University, Auburn, AL, United States of America., Rodning SP; Department of Animal Sciences, Auburn University, Auburn, AL, United States of America., Wang X; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States of America.; HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States of America., Moisá SJ; Department of Animal Sciences, University of Tennessee, Knoxville, TN, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jul 26; Vol. 19 (7), pp. e0306431. Date of Electronic Publication: 2024 Jul 26 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0306431
Abstrakt: The objective of our study was to evaluate the effect of endophyte-infected tall fescue (E+) seeds intake on liver tissue transcriptome in growing Angus × Simmental steers and heifers through RNA-seq analysis. Normal weaned calves (~8 months old) received either endophyte-free tall fescue (E-; n = 3) or infected tall fescue (E+; n = 6) seeds for a 30-d period. The diet offered was ad libitum bermudagrass (Cynodon dactylon) hay combined with a nutritional supplement of 1.61 kg (DM basis) of E+ or E- tall fescue seeds, and 1.61 kg (DM basis) of energy/protein supplement pellets for a 30-d period. Dietary E+ tall fescue seeds were included in a rate of 20 μg of ergovaline/kg BW/day. Liver tissue was individually obtained through biopsy at d 30. After preparation and processing of the liver samples for RNA sequencing, we detected that several metabolic pathways were activated (i.e., upregulated) by the consumption of E+ tall fescue. Among them, oxidative phosphorylation, ribosome biogenesis, protein processing in endoplasmic reticulum and apoptosis, suggesting an active mechanism to cope against impairment in normal liver function. Interestingly, hepatic protein synthesis might increase due to E+ consumption. In addition, there was upregulation of "thermogenesis" KEGG pathway, showing a possible increase in energy expenditure in liver tissue due to consumption of E+ diet. Therefore, results from our study expand the current knowledge related to liver metabolism of growing beef cattle under tall fescue toxicosis.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
(Copyright: © 2024 Alfaro et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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