| Autor: |
Sharma M; Institute of Nano Science and Technology, Sector-81, Mohali, Punjab-140306, India. tapasi@inst.ac.in., Kaur C; Institute of Nano Science and Technology, Sector-81, Mohali, Punjab-140306, India. tapasi@inst.ac.in., Singhmar P; Institute of Nano Science and Technology, Sector-81, Mohali, Punjab-140306, India. tapasi@inst.ac.in., Rai S; Institute of Nano Science and Technology, Sector-81, Mohali, Punjab-140306, India. tapasi@inst.ac.in., Sen T; Institute of Nano Science and Technology, Sector-81, Mohali, Punjab-140306, India. tapasi@inst.ac.in. |
| Abstrakt: |
The convergence of DNA origami and surface-enhanced Raman spectroscopy (SERS) has opened a new avenue in bioanalytical sciences, particularly in the detection of single-molecule proteins. This breakthrough has enabled the development of advanced sensor technologies for diagnostics. DNA origami offers a highly controllable framework for the precise positioning of nanostructures, resulting in superior SERS signal amplification. In our investigation, we have successfully designed and synthesized DNA origami-based gold nanorod monomer and dimer assemblies. Moreover, we have evaluated the potential of dimer assemblies for label-free detection of a single biomolecule, namely epidermal growth factor receptor (EGFR), a crucial biomarker in cancer research. Our findings have revealed that the significant Raman amplification generated by DNA origami-assembled gold nanorod dimer nanoantennas facilitates the label-free identification of Raman peaks of single proteins, which is a prime aim in biomedical diagnostics. The present work represents a significant advancement in leveraging plasmonic nanoantennas to realize single protein SERS for the detection of various cancer biomarkers with single-molecule sensitivity. |
