ER-transiting bacterial toxins amplify STING innate immune responses and elicit ER stress.

Autor: Schlenker C; Program in Oncology University of Maryland, Baltimore (UMB), School of Medicine , Baltimore, Maryland, USA., Richard K; Department of Microbiology and Immunology, School of Medicine , Baltimore, Maryland, USA., Skobelkina S; Program in Oncology University of Maryland, Baltimore (UMB), School of Medicine , Baltimore, Maryland, USA., Mathena RP; Department of Microbiology and Immunology, School of Medicine , Baltimore, Maryland, USA., Perkins DJ; Program in Oncology University of Maryland, Baltimore (UMB), School of Medicine , Baltimore, Maryland, USA.; Department of Microbiology and Immunology, School of Medicine , Baltimore, Maryland, USA.
Jazyk: angličtina
Zdroj: Infection and immunity [Infect Immun] 2024 Aug 13; Vol. 92 (8), pp. e0030024. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1128/iai.00300-24
Abstrakt: The cGAS/STING sensor system drives innate immune responses to intracellular microbial double-stranded DNA (dsDNA) and bacterial cyclic nucleotide second messengers (e.g., c-di-AMP). STING-dependent cell-intrinsic responses can increase resistance to microbial infection and speed pathogen clearance. Correspondingly, STING activation and signaling are known to be targeted for suppression by effectors from several bacterial pathogens. Whether STING responses are also positively regulated through sensing of specific bacterial effectors is less clear. We find that STING activation through dsDNA, by its canonical ligand 2'-3' cGAMP, or the small molecule DMXAA is potentiated following intracellular delivery of the AB 5 toxin family member pertussis toxin from Bordetella pertussis or the B subunit of cholera toxin from Vibrio cholerae . Entry of pertussis toxin or cholera toxin B into mouse macrophages triggers markers of endoplasmic reticulum (ER) stress and enhances ligand-dependent STING responses at the level of STING receptor activation in a manner that is independent of toxin enzymatic activity. Our results provide an example in which STING responses integrate information about the presence of relevant ER-transiting bacterial toxins into the innate inflammatory response and may help to explain the in vivo adjuvant effects of catalytically inactive toxins.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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