Enhancing the Physical, Antimicrobial, and Osteo/Odontogenic Properties of a Sol-Gel-Derived Tricalcium Silicate by Graphene Oxide for Vital Pulp Therapies.

Autor:	Abdalla MM; Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.; Dental Biomaterials, Faculty of Dental Medicine, Al-Azhar University, Cairo 11651, Egypt., Nizami MZI; ADA, Forsyth Institute, Cambridge, MA 02142, USA., Rajasekar V; Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China., Basabrain M; Restorative Dental Sciences, Faculty of Dentistry, Umm Al-Qura University, Makkah 24382, Saudi Arabia., Lung CYK; Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China., Yiu CKY; Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Jazyk:	angličtina
Zdroj:	Journal of functional biomaterials [J Funct Biomater] 2024 Jul 13; Vol. 15 (7). Date of Electronic Publication: 2024 Jul 13.
DOI:	10.3390/jfb15070193
Abstrakt:	Objectives: This study developed a sol-gel tricalcium silicate/graphene oxide (TCS-GO) composite and examined its physicochemical properties, antimicrobial activity, and osteo/odontogenic effect on dental pulp stem cells. Methods: Tricalcium silicate was synthesized and combined with graphene oxide at three different concentrations, namely 0.02%, 0.04%, and 0.08% w / w , while tricalcium silicate and mineral trioxide aggregate served as controls. The setting time, compressive strength, pH, and calcium ion release of the composites were evaluated, as well as antimicrobial properties against Streptococcus mutans and Lactobacillus acidophilus. Additionally, the viability of dental pulp stem cells; apatite forming ability; and the gene expression of Alkaline phosphatase, Dentin sialophosphoprotein, and Runt-related transcription factor 2 were assessed. Results: TCS-GO (0.08%) showed a significantly shorter setting time and higher compressive strength when compared to MTA ( p < 0.05). Additionally, tricalcium silicate and TCS-GO groups showed a higher release of Ca ions than MTA, with no significant difference in pH values among the different groups. TCS-GO (0.08%) also demonstrated a significantly stronger antimicrobial effect against Lactobacillus acidophilus compared to MTA ( p < 0.05). ALP expression was higher in TCS-GO (0.08%) than MTA on days 3 and 7, while DSPP expression was higher in TCS-GO (0.08%) than MTA on day 3 but reversed on day 7. There was no significant difference in RUNX2 expression between TCS-GO (0.08%) and MTA on days 3 and 7. Conclusions: The TCS-GO (0.08%) composite demonstrated superior physicochemical characteristics and antimicrobial properties compared to MTA. Moreover, the early upregulation of ALP and DSPP markers in TCS-GO (0.08%) indicates that it has the potential to promote and enhance the osteo/odontogenic differentiation of DPSCs.
Databáze:	MEDLINE
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