Target Enzymes of Origanum majorana and Rosmarinus officinalis Essential Oils in Black Cutworm ( Agrotis ipsilon ): In Vitro and In Silico Studies.

Autor: Ahmed FS; Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza 12613, Egypt., Helmy WS; Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza 12613, Egypt., Alfuhaid NA; Department of Biology, College of Science and Humanities, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia., Moustafa MAM; Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Jazyk: angličtina
Zdroj: Insects [Insects] 2024 Jun 28; Vol. 15 (7). Date of Electronic Publication: 2024 Jun 28.
DOI: 10.3390/insects15070483
Abstrakt: In this study, in vitro and in silico approaches were employed to assess the toxicity of marjoram ( Origanum majorana ) and rosemary ( Rosmarinus officinalis ) essential oils (EOs) to A. ipsilon larvae. The study determined the activities of ATPases in the larvae after treatment with the LC 20 and LC 70 of each EO. α-esterase and glutathione-S-transferase (GST) activities were also determined after treatment with LC 10 and LC 30 of each EO. Furthermore, molecular docking was employed to determine the binding affinity of terpinene-4-ol and α-pinene, the major constituents of O. majorana , and R. officinalis EOs, respectively, compared to the co-crystallized ligand of α-esterase, diethyl hydrogen phosphate (DPF). Toxicity assays revealed that O. majorana EO was more toxic than R. officinalis EO to the A. ipsilon larvae at 96 h post-treatment. However, the LC 20 and LC 70 of the latter significantly inhibited the activity of the Na + -K + pump at almost all intervals. The same concentrations significantly inhibited the Mg 2+ /Ca 2+ -ATPase and Ca 2+ pump at 96 h post-treatment. In contrast, O. majorana EO showed a variable effect on the Na+-K+ pump across different time intervals. On the other hand, LC 10 and LC 30 of both EOs showed varied effects on α-esterase and GST over time. Molecular docking revealed energy scores of -4.51 and -4.29 kcal/mol for terpinene-4-ol and α-pinene, respectively, compared to a score of -4.67 for PDF. Our study demonstrated the toxicity of the tested EOs to A. ipsilon , suggesting their potential efficacy as insecticides.
Databáze: MEDLINE
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