| Autor: |
Dinu S; Department of Pedodontics, Faculty of Dental Medicine, 'Victor Babeș' University of Medicine and Pharmacy, No. 9, Revolutiei 1989 Bv., 300041 Timisoara, Romania.; Pediatric Dentistry Research Center, Faculty of Dental Medicine, Victor Babeș University of Medicine and Pharmacy, No. 9, Revolutiei Bv., 300041 Timisoara, Romania., Matichescu A; Department of Preventive, Community Dentistry and Oral Health, Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy 'Victor Babeș', 300040 Timisoara, Romania.; Translational and Experimental Clinical Research Centre in Oral Health, 'Victor Babeș' University of Medicine and Pharmacy, 14A Tudor Vladimirescu Ave., 300173 Timișoara, Romania., Buzatu R; Department of Dental Aesthetics, Faculty of Dental Medicine, 'Victor Babeș' University of Medicine and Pharmacy Timisoara, Revolutiei Boulevard 9, 300041 Timisoara, Romania., Marcovici I; Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.; Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, 'Victor Babeș' University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania., Geamantan-Sirbu A; Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.; Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, 'Victor Babeș' University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania., Semenescu AD; Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.; Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, 'Victor Babeș' University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania., Bratu RC; Faculty of Dental Medicine, 'Victor Babeș' University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041 Timișoara, Romania., Bratu DC; Department of Orthodontics II, Faculty of Dental Medicine, 'Victor Babeș' University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041 Timișoara, Romania. |
| Abstrakt: |
Chlorhexidine (CHX) represents one of the most commonly used antiseptics in dentistry and other medical-pharmaceutical fields due to its broad-spectrum antimicrobial activity. However, the potential toxic events arising from its common use in practice has become a subject of increasing concern. Thus, the present study was designed to investigate the potential toxicity of CHX digluconate at concentrations covering its antibacterial properties (0.0002-0.2%) in HGF primary gingival fibroblasts, HaCaT immortalized human keratinocytes, and JB6 Cl 41-5a epidermal cells, as well as its irritant action in ovo. Our results indicate that CHX exerted a concentration- and time-dependent cytotoxicity in all cell lines, which was evidenced by the reduction in cell viability, number, and confluence, damaged cell membrane integrity, impaired cell morphology, and specific apoptotic nuclear shape. The highest cytotoxicity was caused by CHX digluconate 0.02% and 0.2%, concentrations, at which an irritant effect on the chorioallantoic membrane was also observed. The novel findings revealed in this research contribute to the overall safety profile of CHX and stand as a basis for further investigations in this regard. |
