Characterization of a monkey model with experimental retinal damage induced by N-methyl-D-aspartate.

Autor:	Liu G; Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China., Huang L; The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, China., Tan J; Xiamen Eye Center, Xiamen University, Xiamen, 361004, China., Wang Y; Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen, 518040, China., Lan C; The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, China., Chen Y; Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen, 518040, China., Mao Y; The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, China., Wang X; Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen, 518040, China., Fan N; Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Jinan University, Shenzhen, 518040, China., Zhu Y; The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, China., Zhu X; Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.; Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, Sichuan, 610072, China., Liu X; Xiamen Eye Center, Xiamen University, Xiamen, 361004, China.; Department of Ophthalmology, Shenzhen People's Hospital, the 2nd Clinical Medical College, Jinan University, Shenzhen, 518020, China.
Jazyk:	angličtina
Zdroj:	Disease models & mechanisms [Dis Model Mech] 2024 Aug 01; Vol. 17 (8). Date of Electronic Publication: 2024 Jul 26.
DOI:	10.1242/dmm.050033
Abstrakt:	N-methyl-D-aspartate (NMDA)-induced retinal damage has been well studied in rodents, but the detailed mechanisms have not yet been characterized in nonhuman primates. Here, we characterized the retinal degenerative effects of NMDA on rhesus monkeys in vivo. NMDA saline or saline-only control was injected intravitreally to the randomly assigned eyes and contralateral eyes of four rhesus monkeys, respectively. The structural and functional changes of retina were characterized by optical coherence tomography and electroretinography on days 0, 4, 30 and 60 post injection. Both optic discs and macular areas of the NMDA-injected eyes initially presented with a transient retinal thickening, followed by continued retinal thinning. The initial, transient retinal thickening has also been observed in glaucoma patients, but this has not been reported in rodent NMDA models. This initial response was followed by loss of retina ganglion cells (RGCs), which is similar to glaucomatous optic neuropathy and other RGC-related retinal degenerations. The amplitudes of both the photopic negative response and pattern electroretinogram decreased significantly and remained low until the end of the study. Thus, the NMDA monkey model may serve as a more clinically relevant animal model of retinal damage. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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