Influence of genetic polymorphisms of Hg metabolism and DNA repair on the frequencies of micronuclei, nucleoplasmic bridges, and nuclear buds in communities living in gold mining areas.

Autor: Espitia-Pérez L; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia. Electronic address: lydaespitia@unisinu.edu.co., Brango H; Departamento de Matemáticas y Estadística, Universidad del Norte, Barranquilla, Colombia., Peñata-Taborda A; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia., Galeano-Páez C; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia., Jaramillo-García M; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia (Postmorten)., Espitia-Pérez P; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia., Pastor-Sierra K; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia., Bru-Cordero O; Universidad Nacional de Colombia, Dirección académica, kilómetro 9, vía Valledupar-La Paz, La Paz, Cesar, Colombia., Hoyos-Giraldo LS; Department of Biology, Research Group Genetic Toxicology and Cytogenetics, Faculty of Natural Sciences and Education, Universidad del Cauca, Popayán, Cauca, Colombia., Reyes-Carvajal I; Department of Biology, Research Group Genetic Toxicology and Cytogenetics, Faculty of Natural Sciences and Education, Universidad del Cauca, Popayán, Cauca, Colombia., Saavedra-Trujillo D; Department of Biology, Research Group Genetic Toxicology and Cytogenetics, Faculty of Natural Sciences and Education, Universidad del Cauca, Popayán, Cauca, Colombia., Ricardo-Caldera D; Grupo de Investigación Enfermedades Tropicales y Resistencia Bacteriana, Universidad del Sinú, Montería, Córdoba, Colombia., Coneo-Pretelt A; Facultad de Ciencias de la Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad del Sinú, Montería, Córdoba, Colombia.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2024 Jul; Vol. 897, pp. 503790. Date of Electronic Publication: 2024 Jun 13.
DOI: 10.1016/j.mrgentox.2024.503790
Abstrakt: Fishing communities living near gold mining areas are at increased risk of mercury (Hg) exposure via bioaccumulation of methylmercury (MeHg) in fish. This exposure has been linked to health effects that may be triggered by genotoxic events. Genetic polymorphisms play a role in the risk associated with Hg exposure. This study evaluated the effect of single nucleotide polymorphisms (SNPs) in metabolic and DNA repair genes on genetic instability and total hair Hg (T-Hg) levels in 78 individuals from "La Mojana" in northern Colombia and 34 individuals from a reference area. Genetic instability was assessed by the frequency of micronuclei (MNBN), nuclear buds (NBUDS), and nucleoplasmic bridges (NPB). We used a Poisson regression to assess the influence of SNPs on T-Hg levels and genetic instability, and a Bayesian regression to examine the interaction between Hg detoxification and DNA repair. Among exposed individuals, carriers of XRCC1 Arg399Gln had a significantly higher frequency of MNBN. Conversely, the XRCC1 Arg194Trp and OGG1 Ser326Cys polymorphisms were associated with lower frequencies of MNBN. XRCC1 Arg399Gln , XRCC1 Arg280His , and GSTM1 Null carriers showed lower NPB frequencies. Our results also indicated that individuals with the GSTM1 Null and GSTT1 null polymorphisms had a 1.6-fold risk for higher T-Hg levels. The Bayesian model showed increased MNBN frequencies in carriers of the GSTM1 Null polymorphism in combination with XRCC1 Arg399Gln and increased NBUDS frequencies in the GSTM1 Null carriers with the XRCC3 Thr241Met and OGG1 Ser326Cys alleles. The GSTM1 + variant was found to be a protective factor in individuals carrying OGG1 Ser326Cys (MNBN) and XRCC1 Arg280His (NPB); the GSTT1 + polymorphism combined with XRCC Arg194Trp also modulated lower MNBN frequencies, while GSTT1 + carriers with the XRCC1 Arg399Gln allele showed lower NPB frequencies. Consistent with GSTM1, GSTT1 Null carriers with XRCC3 Thr241Met showed increased NBUDS frequency. With the rise of gold mining activities, these approaches are vital to identify and safeguard populations vulnerable to Hg's toxic effects.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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