Detection of sildenafil and its 9 metabolites in a post-race horse urine sample: A case report.

Autor: Kabi L E; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye. Electronic address: erolkabil@gmail.com., Zor TA; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye., Göktaş EF; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye., Güneş E; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye., Yatanaslan L; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye., Tektaş MH; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye., İnceman B; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye., Tufan M; Doping Control Laboratory, Department of Pharmacology, Istanbul Pendik Veterinary Control Institute, Istanbul 34890, Turkiye.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Oct 15; Vol. 249, pp. 116343. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1016/j.jpba.2024.116343
Abstrakt: The use of prohibited substances in horse racing is a major concern that jeopardizes both the fairness of competitions and the health of horses. This problem can stem from the use of licensed drugs for animal health, as well as unlicensed substances. Horse doping laboratories monitor the potential use of these substances in racehorses within the framework of regulations set by the International Federation of Horse Racing Authority. In this context, sildenafil and its major metabolite n-desmethyl sildenafil were detected in a post-race horse urine sample sent to the Pendik Veterinary Control Institute Doping Control Laboratory through a screening analysis performed with Liquid Chromatography Triple Quadrupole Mass Spectrometry. These results were confirmed by Q Exactive Orbitrap Mass Spectrometry and follow-up analyses were performed. As a result of these analyses; simultaneous detection of 9 metabolites in horse urine was reported, two of them for the first time. In addition, the pioneer and comprehensive data resulting from this study provide preliminary data for future studies and anti-doping analyses.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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