Automated Immunoprecipitation Workflow for Comprehensive Acetylome Analysis.
| Autor: | Gritsenko MA; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA., Tsai CF; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA., Kim H; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA., Liu T; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. tao.liu@pnnl.gov. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2823, pp. 173-191. |
| DOI: | 10.1007/978-1-0716-3922-1_12 |
| Abstrakt: | Immunoprecipitation is one of the most effective methods for enrichment of lysine-acetylated peptides for comprehensive acetylome analysis using mass spectrometry. Manual acetyl peptide enrichment method using non-conjugated antibodies and agarose beads has been developed and applied in various studies. However, it is time-consuming and can introduce contaminants and variability that leads to potential sample loss and decreased sensitivity and robustness of the analysis. Here we describe a fast, automated enrichment protocol that enables reproducible and comprehensive acetylome analysis using a magnetic bead-based immunoprecipitation reagent. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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