Just passing through: Deploying aquaporins in microbial cell factories.

Autor: Jenkins Sánchez LR; BioPort Group, Centre for Synthetic Biology, Department of Biotechnology, Faculty of Bio-science Engineering, Ghent University, Ghent, Belgium., Sips LM; BioPort Group, Centre for Synthetic Biology, Department of Biotechnology, Faculty of Bio-science Engineering, Ghent University, Ghent, Belgium., Van Bogaert INA; BioPort Group, Centre for Synthetic Biology, Department of Biotechnology, Faculty of Bio-science Engineering, Ghent University, Ghent, Belgium.
Jazyk: angličtina
Zdroj: Biotechnology progress [Biotechnol Prog] 2024 Jul 25, pp. e3497. Date of Electronic Publication: 2024 Jul 25.
DOI: 10.1002/btpr.3497
Abstrakt: As microbial membranes are naturally impermeable to even the smallest biomolecules, transporter proteins are physiologically essential for normal cell functioning. This makes transporters a key target area for engineering enhanced cell factories. As part of the wider cellular transportome, aquaporins (AQPs) are responsible for transporting small polar solutes, encompassing many compounds which are of great interest for industrial biotechnology, including cell feedstocks, numerous commercially relevant polyols and even weak organic acids. In this review, examples of cell factory engineering by targeting AQPs are presented. These AQP modifications aid in redirecting carbon fluxes and boosting bioconversions either by enhanced feedstock uptake, improved intermediate retention, increasing product export into the media or superior cell viability against stressors with applications in both bacterial and yeast production platforms. Additionally, the future potential for AQP deployment and targeting is discussed, showcasing hurdles and considerations of this strategy as well as recent advances and future directions in the field. By leveraging the natural diversity of AQPs and breakthroughs in channel protein engineering, these transporters are poised to be promising tools capable of enhancing a wide variety of biotechnological processes.
(© 2024 American Institute of Chemical Engineers.)
Databáze: MEDLINE
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