Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community.

Autor: Miguel F; IPS COVID Lab, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal., Baleizão AR; IPS COVID Lab, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal., Gomes AG; IPS COVID Lab, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; RESILIENCE-Center for Regional Resilience and Sustainability, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; MARE-Marine and Environmental Sciences Centre, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Campus do IPS, Estefanilha, 2910-761 Setúbal, Portugal.; Departamento de Engenharia Química e Biológica, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal., Caria H; IPS COVID Lab, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; Departamento de Engenharia Química e Biológica, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; BioISI-Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.; Departamento de Ciências Biomédicas, Escola Superior de Saúde, Instituto Politécnico de Setúbal, Campus do IPS, Estefanilha, 2914-503 Setúbal, Portugal., Serralha FN; RESILIENCE-Center for Regional Resilience and Sustainability, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; Departamento de Engenharia Química e Biológica, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal., Justino MC; IPS COVID Lab, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; RESILIENCE-Center for Regional Resilience and Sustainability, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.; MARE-Marine and Environmental Sciences Centre, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Campus do IPS, Estefanilha, 2910-761 Setúbal, Portugal.; Departamento de Engenharia Química e Biológica, Escola Superior de Tecnologia do Barreiro, Instituto Politécnico de Setúbal, Rua Américo da Silva Marinho, 2839-001 Lavradio, Portugal.
Jazyk: angličtina
Zdroj: Biotech (Basel (Switzerland)) [BioTech (Basel)] 2024 Jul 11; Vol. 13 (3). Date of Electronic Publication: 2024 Jul 11.
DOI: 10.3390/biotech13030026
Abstrakt: Amidst the COVID-19 pandemic, the Polytechnic University of Setúbal (IPS) used its expertise in molecular genetics to establish a COVID-19 laboratory, addressing the demand for community-wide testing. Following standard protocols, the IPS COVID Lab received national accreditation in October 2020 and was registered in February 2021. With the emergence of new SARS-CoV-2 variants and safety concerns for students and staff, the lab was further challenged to develop rapid and sensitive diagnostic technologies. Methodologies such as sample-pooling extraction and multiplex protocols were developed to enhance testing efficiency without compromising accuracy. Through Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR) analysis, the effectiveness of sample pooling was validated, proving to be a clear success in COVID-19 screening. Regarding multiplex analysis, the IPS COVID Lab developed an in-house protocol, achieving a sensitivity comparable to that of standard methods while reducing operational time and reagent consumption. This approach, requiring only two wells of a PCR plate (instead of three for samples), presents a more efficient alternative for future testing scenarios, increasing its throughput and testing capacity while upholding accuracy standards. The lessons learned during the SARS-CoV-2 pandemic provide added value for future pandemic situations.
Databáze: MEDLINE
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