[Regulatory effect of Diwu Yanggan Decoction on lysoglycerophospholipids in circulating exosomes in a mouse model of nonalcoholic fatty liver disease].

Autor:	Chen G; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.; Ezhou Central Hospital, Department of Pharmacy, Ezhou 436000, China., Xiang X; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China., Zeng Z; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China., Huang R; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China., Jin S; School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China., Xiao M; Hepatic Disease Institute, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430000, China., Song C; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.; Hubei Shizhen Laboratory, Wuhan 430065, China.; Center of Traditional Chinese Medicine Modernization for Liver Diseases, Hubei University of Chinese Medicine, Wuhan 430065, China.
Jazyk:	čínština
Zdroj:	Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2024 Jul 20; Vol. 44 (7), pp. 1382-1388.
DOI:	10.12122/j.issn.1673-4254.2024.07.18
Abstrakt:	Objective: To evaluate the regulatory effect of Diwu Yanggan (DWYG) Decoction on lysoglycerophospholipids (Lyso-GPLs) in circulating exosomes in a mouse model of nonalcoholic fatty liver disease (NAFLD). Methods: Circulating exosomes isolated from mouse serum by size exclusion chromatography were morphologically characterized using transmission electron microscope and examined for surface markers CD9, CD63 and TSG101 using Western blotting. Twenty-four male Kunming mice were randomized into 3 groups for normal feeding (control, n =8) or high-fat diet feeding for 1 week to induce NAFLD, after which the latter mice were given DWYG decoction (treatment group, n =8) or normal saline (model group, n =8) by gavage for 4 weeks. After the last treatment, blood samples were collected from the mice for testing serum TC, HDL-C, LDL-C, ALT and AST levels and isolating circulating exosomes. Using multivariate statistical analysis based on targeted metabolomics strategy, the potential biomarkers for Lyso-GPLs in the exosomes were screened. Results: The isolated exosomes about 100 nm in size had a typical saucer-like structure with distinct double-layer membranes and a mean particle size of 137.5 nm and expressed the specific surface marker proteins CD9, CD63 and TSG101. The mouse models of NAFLD had significantly increased serum levels of TC, HDL-C, LDL-C and AST and lowered serum ALT level. A total of 43 Lyso-GPLs with significant reduction after DWYG Decoction treatment were identified in NAFLD mice. Conclusion: DWYG Decoction can regulate Lyso-GPLs in circulating exosomes in NAFLD mice, which provides a new clue for studying the therapeutic mechanism of DWYG Decoction for liver disease.
Databáze:	MEDLINE
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