Bone marrow-derived mesenchymal stem cells reduce CCl 4 -induced kidney injury and fibrosis in male Wistar rats.

Autor: Adel A; Histology, Cell Biology and Genetic Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Abdul-Hamid M; Histology, Cell Biology and Genetic Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Abdel-Kawi SH; Medical Histology and Cell Biology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt., A Abdelaziz M; Basic Medical Sciences Department, College of Medicine, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia.; Medical Physiology Department, Faculty of Medicine, Al-Azhar University, Cairo, Egypt., Sakr HI; Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, Egypt.; Department of Medical Physiology, General Medicine Practice Program, Batterjee Medical College, Jeddah, Saudi Arabia., Ahmed OM; Molecular Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Jazyk: angličtina
Zdroj: Renal failure [Ren Fail] 2024 Dec; Vol. 46 (2), pp. 2319330. Date of Electronic Publication: 2024 Jul 25.
DOI: 10.1080/0886022X.2024.2319330
Abstrakt: Aim: This study explores the possible therapeutic role of rats and mice bone marrow-derived mesenchymal stem cells (BM-MSCs) on renal damage and toxicity brought on by carbon tetrachloride (CCl 4 ) in Wistar rats.
Methods: Following an intraperitoneal injection of CCl 4 (0.5 mL/kg b.w. twice weekly) for eight weeks, male Wistar rats were intravenously treated with rats and mice BM-MSCs (1 × 10 6 cells in 0.2 mL Dulbecco's Modified Eagle Medium (DMEM)/rat/week) a week for four weeks. Kidney functions were evaluated and kidney samples were examined using hematoxylin and eosin (H&E), Masson's trichrome (MT) staining techniques, and electron microscopy analysis. Kidney cyclooxygenase-2 (COX-2), protein 53 (p53), and tumor necrosis factor-α (TNF-α) were detected by immunohistochemical staining techniques. Additionally, bioindicators of oxidative stress and antioxidant defense systems were identified in kidney tissue.
Results: In CCl 4 -injected rats, serum creatinine, urea, and uric acid levels significantly increased, as did renal lipid peroxidation (LPO), while superoxide dismutase, glutathione peroxidase (GPx), glutathione (GSH) transferase, and GSH levels significantly dropped in the kidneys. Histologically, the kidneys displayed a wide range of structural abnormalities, such as glomerular shrinkage, tubular dilations, inflammatory leukocytic infiltration, fibroblast proliferation, and elevated collagen content. Inflammatory cytokines like COX-2 and TNF-α as well as the pro-apoptotic mediator p53 were considerably upregulated. Treatment of BM-MSCs from mice and rats with CCl 4 -injected rats considerably reduced the previously noted abnormalities.
Conclusions: By boosting antioxidant defense and reducing apoptosis and inflammation, BM-MSCs from mice and rats were able to enhance kidney function and histological integrity in rats that had received CCl 4 injections.
Databáze: MEDLINE
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