One- and two-photon brightness of proteins interacting with gold. A closer look at gold-insulin conjugates.

Autor: Bain D; Institut lumière matière, UMR5306, Université Claude Bernard Lyon1-CNRS, Univ. Lyon 69622, Villeurbanne cedex, France. rodolphe.antoine@univ-lyon1.fr., Yuan H; Institut lumière matière, UMR5306, Université Claude Bernard Lyon1-CNRS, Univ. Lyon 69622, Villeurbanne cedex, France. rodolphe.antoine@univ-lyon1.fr., Pniakowska A; Institute of Advanced Materials, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland., Hajda A; Institute of Advanced Materials, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland., Bouanchaud C; Institut lumière matière, UMR5306, Université Claude Bernard Lyon1-CNRS, Univ. Lyon 69622, Villeurbanne cedex, France. rodolphe.antoine@univ-lyon1.fr., Chirot F; Institut lumière matière, UMR5306, Université Claude Bernard Lyon1-CNRS, Univ. Lyon 69622, Villeurbanne cedex, France. rodolphe.antoine@univ-lyon1.fr., Comby-Zerbino C; Institut lumière matière, UMR5306, Université Claude Bernard Lyon1-CNRS, Univ. Lyon 69622, Villeurbanne cedex, France. rodolphe.antoine@univ-lyon1.fr., Gueguen-Chaignon V; Université Claude Bernard Lyon 1, CNRS UAR3444, Inserm US8, ENS de Lyon, SFR Biosciences, Lyon 69007, France., Bonačić-Koutecký V; Center of Excellence for Science and Technology-Integration of Mediterranean Region (STIM) at Interdisciplinary Center for Advanced Sciences and Technology (ICAST), University of Split, Poljička cesta 35, 21000 Split, Croatia., Olesiak-Banska J; Institute of Advanced Materials, Wroclaw University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland., Sanader Maršić Ž; Center of Excellence for Science and Technology-Integration of Mediterranean Region (STIM) at Interdisciplinary Center for Advanced Sciences and Technology (ICAST), University of Split, Poljička cesta 35, 21000 Split, Croatia.; Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia. zsm@pmfst.hr., Antoine R; Institut lumière matière, UMR5306, Université Claude Bernard Lyon1-CNRS, Univ. Lyon 69622, Villeurbanne cedex, France. rodolphe.antoine@univ-lyon1.fr.
Jazyk: angličtina
Zdroj: Nanoscale [Nanoscale] 2024 Aug 13; Vol. 16 (31), pp. 14953-14958. Date of Electronic Publication: 2024 Aug 13.
DOI: 10.1039/d4nr01697a
Abstrakt: Red luminophores displaying large Stokes shift and high-quantum yields are obtained when gold salts are reacted with proteins under strongly alkaline conditions. Although bovine serum albumin (BSA) has mainly been used as a protein template, other attempts to prepare red luminophores have been proposed using other proteins. Here, we report on the structural characterization and nonlinear optical properties of insulin-gold conjugates. Such conjugates display strong luminescence at ∼670 nm with quantum yields that reach 5.4%. They also display long luminescence lifetimes allowing efficient reactive oxygen species generation, with a quantum yield of 1 O 2 generation reaching 13%. In addition, they exhibit remarkable nonlinear optical properties and in particular a strong two-photon excited fluorescence (TPEF) cross section in the range of 800-1100 nm. By combining experimental studies and time-dependent density functional theory simulations (TD-DFT), we show the formation of insulin-Au(III) conjugates. The interaction of Au(III) ions with the aromatic rings of tyrosine induces charge transfer-like excitation in the visible range. Experimental investigations, together with molecular dynamics simulations of insulin and calculations of electronic properties in a model system, are performed to explore the origin of optical features and the structure-optical property relationship, leading the way to new concepts for nonlinear optics using protein-Au(III) conjugates.
Databáze: MEDLINE
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