| Autor: |
Park C; School of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea. nanopark@skku.edu., Lim W; Department of Biomedical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea., Song R; School of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea. nanopark@skku.edu., Han J; School of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea. nanopark@skku.edu., You D; Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences & Technology (SAIHST), Sungkyunkwan University (SKKU), Seoul 06355, Korea., Kim S; Department of Breast Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea., Lee JE; Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences & Technology (SAIHST), Sungkyunkwan University (SKKU), Seoul 06355, Korea.; Division of Breast Surgery, Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medi-cine, Seoul 06351, Korea., van Noort D; Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping 58183, Sweden., Mandenius CF; Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping 58183, Sweden., Lee J; School of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea. nanopark@skku.edu.; Department of Biophysics, Institute of Quantum Biophysics (IQB), Sungkyunkwan University (SKKU), Suwon 16419, Korea., Hyun KA; Department of Mechanical Engineering, Yonsei University, Seoul 03722, Korea., Jung HI; Department of Mechanical Engineering, Yonsei University, Seoul 03722, Korea., Park S; School of Mechanical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea. nanopark@skku.edu.; Department of Biomedical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea.; Department of Biophysics, Institute of Quantum Biophysics (IQB), Sungkyunkwan University (SKKU), Suwon 16419, Korea. |
| Abstrakt: |
Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors. Their size, however, complicates their isolation from mainstream tumor cell populations. Standard techniques like fluorescence-activated cell sorting (FACS) rely on fluorescent labeling, introducing potential challenges in subsequent PGCC analyses. In response, we developed the Isosceles Trapezoidal Spiral Microchannel (ITSμC), a microfluidic device optimizing the Dean drag force ( F D ) and exploiting uniform vortices for enhanced separation. Numerical simulations highlighted ITSμC's advantage in producing robust F D compared to rectangular and standard trapezoidal channels. Empirical results confirmed its ability to segregate larger polystyrene (PS) particles (avg. diameter: 50 μm) toward the inner wall, while directing smaller ones (avg. diameter: 23 μm) outward. Utilizing ITSμC, we efficiently isolated PGCCs from doxorubicin-resistant triple-negative breast cancer (DOXR-TNBC) and patient-derived cancer (PDC) cells, achieving outstanding purity, yield, and viability rates (all greater than 90%). This precision was accomplished without fluorescent markers, and the versatility of ITSμC suggests its potential in differentiating a wide range of heterogeneous cell populations. |
