Blood culture-free ultra-rapid antimicrobial susceptibility testing.

Autor: Kim TH; Bio-MAX Institute, Seoul National University, Seoul, Korea.; Department of Electrical and Computer Engineering, Seoul National University, Seoul, Korea., Kang J; Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Korea.; Integrated Major in Innovative Medical Science, Seoul National University, Seoul, Korea., Jang H; Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Korea., Joo H; Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Korea., Lee GY; Department of Electrical and Computer Engineering, Seoul National University, Seoul, Korea., Kim H; Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Korea., Cho U; QuantaMatrix Inc., Seoul, Korea., Bang H; QuantaMatrix Inc., Seoul, Korea., Jang J; QuantaMatrix Inc., Seoul, Korea., Han S; QuantaMatrix Inc., Seoul, Korea., Kim DY; QuantaMatrix Inc., Seoul, Korea., Lee CM; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea., Kang CK; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea., Choe PG; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea., Kim NJ; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea., Oh MD; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea., Kim TS; Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea., Kim I; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. ihkimmd@snu.ac.kr., Park WB; Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. wbpark1@snu.ac.kr., Kwon S; Bio-MAX Institute, Seoul National University, Seoul, Korea. skwon@snu.ac.kr.; Department of Electrical and Computer Engineering, Seoul National University, Seoul, Korea. skwon@snu.ac.kr.; Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Korea. skwon@snu.ac.kr.; QuantaMatrix Inc., Seoul, Korea. skwon@snu.ac.kr.; Inter-University Semiconductor Research Center, Seoul National University, Seoul, Korea. skwon@snu.ac.kr.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 Aug; Vol. 632 (8026), pp. 893-902. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1038/s41586-024-07725-1
Abstrakt: Treatment assessment and patient outcome for sepsis depend predominantly on the timely administration of appropriate antibiotics 1-3 . However, the clinical protocols used to stratify and select patient-specific optimal therapy are extremely slow 4 . In particular, the major hurdle in performing rapid antimicrobial susceptibility testing (AST) remains in the lengthy blood culture procedure, which has long been considered unavoidable due to the limited number of pathogens present in the patient's blood. Here we describe an ultra-rapid AST method that bypasses the need for traditional blood culture, thereby demonstrating potential to reduce the turnaround time of reporting drug susceptibility profiles by more than 40-60 h compared with hospital AST workflows. Introducing a synthetic beta-2-glycoprotein I peptide, a broad range of microbial pathogens are selectively recovered from whole blood, subjected to species identification or instantly proliferated and phenotypically evaluated for various drug conditions using a low-inoculum AST chip. The platform was clinically evaluated by the enrolment of 190 hospitalized patients suspected of having infection, achieving 100% match in species identification. Among the eight positive cases, six clinical isolates were retrospectively tested for AST showing an overall categorical agreement of 94.90% with an average theoretical turnaround time of 13 ± 2.53 h starting from initial blood processing.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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