Genetic variation in NOTCH1 is associated with overweight and obesity in Brazilian elderly.

Autor: Silva Barcelos EC; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.; Institute of Hematology-Centro di Ricerca Emato-Oncologica (CREO), Department of Medicine and Surgery, University of Perugia, Perugia, Italy., Naslavsky MS; Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil.; Human Genome and Stem Cell Research Center, University of São Paulo, São Paulo, Brazil., Fernandes IS; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Scliar MO; Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil.; Human Genome and Stem Cell Research Center, University of São Paulo, São Paulo, Brazil., Yamamoto GL; Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil., Wang JYT; Human Genome and Stem Cell Research Center, University of São Paulo, São Paulo, Brazil., Bride L; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., de Sousa VP; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Pimassoni LHS; School of Sciences of Santa Casa de Misericórdia de Vitória (EMESCAM), Vitória, Espírito Santo, Brazil., Sportoletti P; Institute of Hematology-Centro di Ricerca Emato-Oncologica (CREO), Department of Medicine and Surgery, University of Perugia, Perugia, Italy., de Paula F; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.; Department of Biological Sciences, Federal University of Espírito Santo, Avenida Fernando Ferrari, 514, Edifício Lídia Behar, Sala 105, Vitória, Espírito Santo, 29075-910, Brazil., von Zeidler SV; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil.; Department of Pathology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil., Duarte YAO; School of Nursing, University of São Paulo, São Paulo, Brazil.; School of Public Health, University of São Paulo, São Paulo, Brazil., Passos-Bueno MR; Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil.; Human Genome and Stem Cell Research Center, University of São Paulo, São Paulo, Brazil., Zatz M; Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil.; Human Genome and Stem Cell Research Center, University of São Paulo, São Paulo, Brazil., Errera FIV; Postgraduate Program in Biotechnology, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil. flavia.valle@ufes.br.; Department of Biological Sciences, Federal University of Espírito Santo, Avenida Fernando Ferrari, 514, Edifício Lídia Behar, Sala 105, Vitória, Espírito Santo, 29075-910, Brazil. flavia.valle@ufes.br.; Postgraduate Program in Biochemistry, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil. flavia.valle@ufes.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 24; Vol. 14 (1), pp. 17096. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1038/s41598-024-65771-1
Abstrakt: Excessive weight (overweight and obesity) is a common disorder involving genetic and environmental factors, associated with cardiovascular diseases, type-2 diabetes, and others. NOTCH1 is critical for the maintenance of stem cells and adult tissues, being reported as a key player in metabolism and adipogenesis in animals. Thus, we test the hypothesis that NOTCH1 Single Nucleotide Polymorphisms (SNPs) are associated with excessive weight. Participants from the census-based cohort SABE (Saúde, Bem Estar e Envelhecimento-Health, Well-Being, and Aging), carried out in the city of São Paulo-Brazil, were stratified into cases and controls according to BMI. We filter the SNPs located at the start and end positions of NOTCH1 and 50 Kb on both sides. We selected SNPs with minor allelic frequency (MAF) greater than or equal to 0.01 and Hardy-Weinberg equilibrium (p > 0.05) and r 2  ≥ 0.8. We performed an association study with genotypes and haplotypes, as well as in silico functional analysis of the identified SNPs. We observed an association of the SNP rs9411207 with the risk of excessive weight, under log-additive model, and the genotype distribution showed an increased frequency of homozygous TT (OR 1.50, CI 1.20-1.88; p = 0.0002). The haplotype GAT constructed from this and other SNPs in high Linkage Disequilibrium was more frequent in excessive-weight individuals (p = 0.003). In silico analyses suggested that these SNPs are likely to affect the transcription of NOTCH1 and other genes involved in adipogenesis and metabolism. This is the first study reporting association between NOTCH1 SNPs and the risk of excessive weight. Considering the possibility of NOTCH1 modulation, additional population studies are important to replicate these data and confirm the usefulness of risk genotypes for management strategies of excessive weight.
(© 2024. The Author(s).)
Databáze: MEDLINE
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