Second harmonic generation microscopy, biaxial mechanical tests and fiber dispersion models in human skin biomechanics.

Autor: Alberini R; Department of Engineering and Architecture, University of Parma, Parma, Italy., Spagnoli A; Department of Engineering and Architecture, University of Parma, Parma, Italy. Electronic address: spagnoli@unipr.it., Sadeghinia MJ; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Trondheim, Norway., Skallerud B; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Trondheim, Norway., Terzano M; Institute of Biomechanics, Graz University of Technology, Graz, Austria., Holzapfel GA; Department of Structural Engineering, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; Institute of Biomechanics, Graz University of Technology, Graz, Austria.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2024 Sep 01; Vol. 185, pp. 266-280. Date of Electronic Publication: 2024 Jul 22.
DOI: 10.1016/j.actbio.2024.07.026
Abstrakt: Advanced numerical simulations of the mechanical behavior of human skin require thorough calibration of the material's constitutive models based on experimental ex vivo mechanical tests along with images of tissue microstructure for a variety of biomedical applications. In this work, a total of 14 human healthy skin samples and 4 additional scarred skin samples were experimentally analyzed to gain deep insights into the biomechanics of human skin. In particular, second harmonic generation (SHG) microscopy was used to extract detailed images of the distribution of collagen fibers, which were subsequently processed using a three-dimensional Fourier transform-based method recently proposed by the authors to quantify the distribution of fiber orientations. Mechanical tests under both biaxial and uniaxial loading were performed to calibrate the relevant mechanical parameters of two widely used constitutive models of soft fiber-reinforced biological tissues that account for non-symmetrical fiber dispersion. The calibration of the models allowed us to identify correlations between the mechanical parameters of the constitutive models considered. STATEMENT OF SIGNIFICANCE: Constitutive models for soft collagenous tissues can accurately reproduce the complex nonlinear and anisotropic mechanical behavior of skin. However, a comprehensive analysis of both microstructural and mechanical parameters is still missing for human skin. In this study, these parameters are determined by combining biaxial mechanical tests and SHG stacks of collagen fibers on ex vivo healthy human skin samples. The constitutive parameters are provided for two widely used hyperelastic models and enable accurate characterization of skin mechanical behavior for advanced numerical simulations.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: