Effects of proanthocyanidin-functionalized hydroxyapatite nanoparticles on dentin bonding.

Autor: Enrich-Essvein T; Department of Operative Dentistry, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Maximo s/n, Granada, E-18071, Spain. tattiana@ugr.es.; Department of Mineralogy and Petrology, Faculty of Sciences, University of Granada, Granada, Spain. tattiana@ugr.es., González-López S; Department of Operative Dentistry, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Maximo s/n, Granada, E-18071, Spain., Rodríguez-Navarro AB; Department of Mineralogy and Petrology, Faculty of Sciences, University of Granada, Granada, Spain., Cifuentes-Jiménez C; Department of Operative Dentistry, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Maximo s/n, Granada, E-18071, Spain., Maravic T; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy., Mazzitelli C; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy., Checchi V; Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy., Josic U; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy., Mazzoni A; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy., Breschi L; Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2024 Jul 24; Vol. 28 (8), pp. 444. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1007/s00784-024-05836-7
Abstrakt: Objectives: To evaluate the effect of proanthocyanidin-functionalized hydroxyapatite nanoparticles (nHAp_PA) used as pretreatment at different concentrations on the microtensile bond strength (µTBS) and endogenous enzymatic activity (MMPs) on pH-cycled dentin after 24 h and 6 months of artificial aging.
Materials and Methods: Fifty human sound dentin blocks were randomly assigned to 5 groups (n = 10): (i) negative control (no treatment); (ii) positive control (pH-cycling); (iii) pH-cycling + 2% nHAp_PA for 60s; (iv) pH-cycling + 6.5% nHAp_PA for 60s; (v) pH-cycling + 15% nHAp_PA for 60s. A self-etch adhesive was used for bonding procedures before resin composite build-ups. Specimens were tested with the µTBS test after 24 h and 6 months of laboratory storage. The proteolytic activity in each group was evaluated with gelatin zymography and in situ zymography. Data were statistically analyzed (p < 0.05).
Results: At 24 h, the µTBS of the experimental groups were significantly higher than the controls (p ≤ 0.001), and no differences were observed between different concentrations (p > 0.05). Artificial aging significantly decreased bond strength in all groups (p ≤ 0.008); however, nHAp_PA 2% still yielded higher bonding values than controls (p ≤ 0.007). The groups pretreated with nHAp_PA exhibited lower MMP-9 and MMP-2 activities compared to the positive control group and almost the same enzymatic activity as the negative control group. In situ zymography showed that after 6 months of aging, nHAp_PA 2% and nHAp_PA 6,5% decreased enzymatic activity as well as the negative control.
Conclusions: Dentin pretreatment with nHAp_PA increased the bonding performance of a self-etch adhesive and decreased MMP-2 and MMP-9 activities after 6 months.
(© 2024. The Author(s).)
Databáze: MEDLINE
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