Metabolomics analysis of human spermatozoa reveals impaired metabolic pathways in asthenozoospermia.

Autor: Guerra-Carvalho B; LAQV-REQUIMTE and Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.; ICBAS-School of Medicine and Biomedical Sciences, University of Porto, Porto, Portugal., Carrageta DF; Clinical and Experimental Endocrinology, UMIB-Unit for Multidisciplinary Research in Biomedicine, ICBAS - School of Medicine and Biomedical Sciences, University of Porto, Porto, Portugal.; Laboratory for Integrative and Translational Research in Population Health (ITR), University of Porto, Porto, Portugal.; Institute of Biomedicine and (iBiMED), Department of Medical Sciences, University of Aveiro, Campus de Santiago Agra do Crasto, Aveiro, Portugal., Maurício T; Institute of Biomedicine and (iBiMED), Department of Medical Sciences, University of Aveiro, Campus de Santiago Agra do Crasto, Aveiro, Portugal.; Mass Spectrometry Centre, LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal., Pereira SC; LAQV-REQUIMTE and Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.; ICBAS-School of Medicine and Biomedical Sciences, University of Porto, Porto, Portugal., Barros A; Department of Pathology, Faculty of Medicine, University of Porto, Porto, Portugal.; Centre for Reproductive Genetics Professor Alberto Barros, Porto, Portugal.; i3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal., Carvalho RA; Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal.; REQUIMTE/LAQV, Group of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal., Alves MG; Institute of Biomedicine and (iBiMED), Department of Medical Sciences, University of Aveiro, Campus de Santiago Agra do Crasto, Aveiro, Portugal., Domingues P; Mass Spectrometry Centre, LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal., Oliveira PF; LAQV-REQUIMTE and Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
Jazyk: angličtina
Zdroj: European journal of clinical investigation [Eur J Clin Invest] 2024 Nov; Vol. 54 (11), pp. e14289. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1111/eci.14289
Abstrakt: Background: Infertility is a major health issue, affecting 15% of reproductive-age couples with male factors contributing to 50% of cases. Asthenozoospermia (AS), or low sperm motility, is a common cause of male infertility with complex aetiology, involving genetic and metabolic alterations, inflammation and oxidative stress. However, the molecular mechanisms behind low motility are unclear. In this study, we used a metabolomics approach to identify metabolic biomarkers and pathways involved in sperm motility.
Methods: We compared the metabolome and lipidome of spermatozoa of men with normozoospermia (n = 44) and AS (n = 22) using untargeted LC-MS and the metabolome of seminal fluid using 1 H-NMR. Additionally, we evaluated the seminal fluid redox status to assess the oxidative stress in the ejaculate.
Results: We identified 112 metabolites and 209 lipids in spermatozoa and 27 metabolites in the seminal fluid of normozoospermic and asthenozoospermic men. PCA analysis of the spermatozoa's metabolomics and lipidomics data showed a clear separation between groups. Spermatozoa of asthenozoospermic men presented lower levels of several amino acids, and increased levels of energetic substrates and lysophospholipids. However, the metabolome and redox status of the seminal fluid was not altered inAS.
Conclusions: Our results indicate impaired metabolic pathways associated with redox homeostasis and amino acid, energy and lipid metabolism in AS. Taken together, these findings suggest that the metabolome and lipidome of human spermatozoa are key factors influencing their motility and that oxidative stress exposure during spermatogenesis or sperm maturation may be in the aetiology of decreased motility in AS.
(© 2024 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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