Assessing immune phenotypes using simple proxy measures: promise and limitations.

Autor:	Downie AE; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA., Barre RS; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.; Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health Sciences Center at San Antonio; San Antonio, TX, USA., Robinson A; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA., Yang J; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA., Chen YH; Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine; New York, NY, USA.; Department of Microbiology, New York University Grossman School of Medicine; New York, NY, USA.; Institute of Biomedical Sciences, Academia Sinica, Taipei City, Taiwan., Lin JD; Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City, Taiwan.; Center for Computational and Systems Biology, National Taiwan University, Taipei City, Taiwan., Oyesola O; Laboratory of Parasitic Diseases, National Institute for Allergy and Infectious Diseases, National Institutes of Health; Bethesda, MD, USA., Yeung F; Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine; New York, NY, USA., Cadwell K; Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine; New York, NY, USA.; Department of Microbiology, New York University Grossman School of Medicine; New York, NY, USA.; Division of Gastroenterology and Hepatology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA., Loke P; Kimmel Center for Biology and Medicine at the Skirball Institute, New York University Grossman School of Medicine; New York, NY, USA.; Department of Microbiology, New York University Grossman School of Medicine; New York, NY, USA.; Laboratory of Parasitic Diseases, National Institute for Allergy and Infectious Diseases, National Institutes of Health; Bethesda, MD, USA., Graham AL; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.; Santa Fe Institute; Santa Fe, NM, USA.
Jazyk:	angličtina
Zdroj:	Discovery immunology [Discov Immunol] 2024 Jun 28; Vol. 3 (1), pp. kyae010. Date of Electronic Publication: 2024 Jun 28 (Print Publication: 2024).
DOI:	10.1093/discim/kyae010
Abstrakt:	The study of immune phenotypes in wild animals is beset by numerous methodological challenges, with assessment of detailed aspects of phenotype difficult to impossible. This constrains the ability of disease ecologists and ecoimmunologists to describe immune variation and evaluate hypotheses explaining said variation. The development of simple approaches that allow characterization of immune variation across many populations and species would be a significant advance. Here we explore whether serum protein concentrations and coarse-grained white blood cell profiles, immune quantities that can easily be assayed in many species, can predict, and therefore serve as proxies for, lymphocyte composition properties. We do this in rewilded laboratory mice, which combine the benefits of immune phenotyping of lab mice with the natural context and immune variation found in the wild. We find that easily assayed immune quantities are largely ineffective as predictors of lymphocyte composition, either on their own or with other covariates. Immunoglobulin G (IgG) concentration and neutrophil-lymphocyte ratio show the most promise as indicators of other immune traits, but their explanatory power is limited. Our results prescribe caution in inferring immune phenotypes beyond what is directly measured, but they do also highlight some potential paths forward for the development of proxy measures employable by ecoimmunologists. Competing Interests: K.C. has received research support from Pfizer, Takeda, Pacific Biosciences, Genentech, and Abbvie, and P.L. has received research funding from Pfizer. K.C. has consulted for or received an honoraria from Puretech Health, Genentech, and Abbvie. K.C. is an inventor on U.S. patent 10,722,600 and provisional patents 62/935,035 and 63/157,225. P.L. is a federal employee. The other authors declare that they have no competing interests. (© The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology.)
Databáze:	MEDLINE
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