Staphylococcus aureus associated with surgical site infections in Western Kenya reveals genomic hotspots for pathogen evolution.

Autor:	Mogoi NN; Department of Biological sciences, Masinde Muliro University of Science and Technology, Kakamega, Kenya., Sifuna AW; Department of Medical Biochemistry, Masinde Muliro University of Science and Technology, Kakamega, Kenya., Okoth PK; Department of Biological sciences, Masinde Muliro University of Science and Technology, Kakamega, Kenya., Reva O; Department of Biochemistry, Genetics and Microbiology, Centre For Bioinformatics And Computational Biology, University of Pretoria, Pretoria, South Africa., Malaba R; Kakamega County General Teaching and Referral Hospital, Kakamega, Kenya., Negesa R; Kakamega County General Teaching and Referral Hospital, Kakamega, Kenya., Nyongesa KP; Department of Medical Microbiology and Parasitology, Masinde Muliro University of Science and Technology, Kakamega, Kenya., Osoro KE; Department of Medical Biochemistry, Masinde Muliro University of Science and Technology, Kakamega, Kenya., Welch M; Department of Biochemistry, University of Cambridge, Cambridge, UK.
Jazyk:	angličtina
Zdroj:	Access microbiology [Access Microbiol] 2024 Jun 27; Vol. 6 (6). Date of Electronic Publication: 2024 Jun 27 (Print Publication: 2024).
DOI:	10.1099/acmi.0.000734.v4
Abstrakt:	Objectives. Staphylococcus aureus is one of the most common pathogens attributed to hospital infections. Although S. aureus infections have been well studied in developed countries, far less is known about the biology of the pathogen in sub-Saharan Africa. Methods. Here, we report on the isolation, antibiotic resistance profiling, whole genome sequencing, and genome comparison of six multi-drug resistant isolates of S. aureus obtained from a referral hospital in Kakamega, Western Kenya. Results. Five of the six isolates contained a 20.7 kb circular plasmid carrying blaZ (associated with resistance to β-lactam antibiotics). These five strains all belonged to the same sequence type, ST152. Despite the similarity of the plasmid in these isolates, whole genome sequencing revealed that the strains differed, depending on whether they were associated with hospital-acquired or community-acquired infections. Conclusion. The intriguing finding is that the hospital-acquired and the community-acquired isolates of S. aureus belonging to the same genotype, ST152, formed two separate sub-clusters in the phylogenetic tree and differed by the repertoire of accessory virulence genes. These data suggest ongoing adaptive evolution and significant genomic plasticity. Competing Interests: The authors declare no conflict of interest. (Copyright © 2024 The Authors.)
Databáze:	MEDLINE
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