Defining a core breath profile for healthy, non-human primates.

Autor: Bobak CA; Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA.; Department of Biomedical Data Science, Dartmouth Geisel School of Medicine, Lebanon, NH, USA., Stevenson KAJM; Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, V6T 1Z3, Canada.; Department of Chemistry, University of Winnipeg, Winnipeg, MB, Canada., Sun N; School of Biomedical Engineering, University of British Columbia, Vancouver, V6T 1Z3, Canada., Khan MS; Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA.; Cargill Inc., Wayzata, MN, USA., Azmir J; Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA., Beccaria M; Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA.; Department of Chemical, Pharmaceutical, and Agricultural Sciences, University of Ferrara, 44121, Ferrara, Italy., Tomko JA; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Fillmore D; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Scanga CA; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Lin PL; Department of Pediatrics, Division of Infectious Disease, Children's Hospital of UPMC, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Flynn JL; Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Hill JE; Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, V6T 1Z3, Canada. jane.hill@ubc.ca.; Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA. jane.hill@ubc.ca.; School of Biomedical Engineering, University of British Columbia, Vancouver, V6T 1Z3, Canada. jane.hill@ubc.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 23; Vol. 14 (1), pp. 17031. Date of Electronic Publication: 2024 Jul 23.
DOI: 10.1038/s41598-024-64910-y
Abstrakt: Non-human primates remain the most useful and reliable pre-clinical model for many human diseases. Primate breath profiles have previously distinguished healthy animals from diseased, including non-human primates. Breath collection is relatively non-invasive, so this motivated us to define a healthy baseline breath profile that could be used in studies evaluating disease, therapies, and vaccines in non-human primates. A pilot study, which enrolled 30 healthy macaques, was conducted. Macaque breath molecules were sampled into a Tedlar bag, concentrated onto a thermal desorption tube, then desorbed and analyzed by comprehensive two-dimensional gas chromatography-time of flight mass spectrometry. These breath samples contained 2,017 features, of which 113 molecules were present in all breath samples. The core breathprint was dominated by aliphatic hydrocarbons, aromatic compounds, and carbonyl compounds. The data were internally validated with additional breath samples from a subset of 19 of these non-human primates. A critical core consisting of 23 highly abundant and invariant molecules was identified as a pragmatic breathprint set, useful for future validation studies in healthy primates.
(© 2024. The Author(s).)
Databáze: MEDLINE
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