| Autor: |
Fabrizi I; Univ. Lille, CNRS UAR 3290─MSAP─Miniaturisation pour la Synthèse, l'Analyse et la Protéomique, Lille F-59000, France., Flament S; Univ. Lille, CNRS UAR 3290─MSAP─Miniaturisation pour la Synthèse, l'Analyse et la Protéomique, Lille F-59000, France., Delhon C; Université Côte d'Azur, CNRS, CEPAM (UMR 7264), Nice F-06300, France., Gourichon L; Université Côte d'Azur, CNRS, CEPAM (UMR 7264), Nice F-06300, France., Vuillien M; Université Côte d'Azur, CNRS, CEPAM (UMR 7264), Nice F-06300, France., Oueslati T; Univ. Lille, CNRS UMR 8164─HALMA─Histoire, Archéologie et Littérature des Mondes Anciens, Lille F-59000, France., Auguste P; Univ. Lille, CNRS UMR 8198─EEP─Evolution, Ecology and Paleontology, Lille F-59000, France., Rolando C; Univ. Lille, CNRS UAR 3290─MSAP─Miniaturisation pour la Synthèse, l'Analyse et la Protéomique, Lille F-59000, France.; Shrieking Sixties, Villeneuve d'Ascq F-59650, France., Bray F; Univ. Lille, CNRS UAR 3290─MSAP─Miniaturisation pour la Synthèse, l'Analyse et la Protéomique, Lille F-59000, France. |
| Abstrakt: |
Collagen from paleontological bones is an important organic material for isotopic measurement, radiocarbon analysis, and paleoproteomic analysis to provide information on diet, dating, taxonomy, and phylogeny. Current paleoproteomic methods are destructive and require from a few milligrams to several tens of milligrams of bone for analysis. In many cultures, bones are raw materials for artifacts that are conserved in museums, which hampers damage to these precious objects during sampling. Here, we describe a low-invasive sampling method that identifies collagen, taxonomy, and post-translational modifications from Holocene and Upper Pleistocene bones dated to 130,000 and 150 BC using dermatological skin tape discs for sampling. The sampled bone micropowders were digested following our highly optimized enhanced filter-aided sample preparation protocol and then analyzed by MALDI FTICR MS and LC-MS/MS for identifying the genus taxa of the bones. We show that this low-invasive sampling does not deteriorate the bones and achieves results similar to those obtained by more destructive sampling. Moreover, this sampling method can be carried out at archeological sites or in museums. |
