Neisserial adhesin A (NadA) binds human Siglec-5 and Siglec-14 with high affinity and promotes bacterial adhesion/invasion.
Autor: | Benucci B; GSK, Siena, Italy., Spinello Z; GSK, Siena, Italy., Calvaresi V; GSK, Siena, Italy.; Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark., Viviani V; GSK, Siena, Italy., Perrotta A; GSK, Siena, Italy.; Università di Siena, Siena, Italy., Faleri A; GSK, Siena, Italy., Utrio Lanfaloni S; GSK, Siena, Italy., Pansegrau W; GSK, Siena, Italy., d'Alterio L; GSK, Siena, Italy., Bartolini E; GSK, Siena, Italy., Pinzuti I; GSK, Siena, Italy., Sampieri K; GSK, Siena, Italy., Giordano A; GSK, Siena, Italy., Rappuoli R; GSK, Siena, Italy., Pizza M; GSK, Siena, Italy., Masignani V; GSK, Siena, Italy., Norais N; GSK, Siena, Italy., Maione D; GSK, Siena, Italy., Merola M; GSK, Siena, Italy.; Università di Napoli Federico II, Naples, Italy. |
---|---|
Jazyk: | angličtina |
Zdroj: | MBio [mBio] 2024 Aug 14; Vol. 15 (8), pp. e0110724. Date of Electronic Publication: 2024 Jul 23. |
DOI: | 10.1128/mbio.01107-24 |
Abstrakt: | Neisserial adhesin A (NadA) is a meningococcal surface protein included as recombinant antigen in 4CMenB, a protein-based vaccine able to induce protective immune responses against Neisseria meningitidis serogroup B (MenB). Although NadA is involved in the adhesion/invasion of epithelial cells and human myeloid cells, its function in meningococcal physiology is still poorly understood. To clarify the role played by NadA in the host-pathogen interaction, we sought to identify its cellular receptors. We screened a protein microarray encompassing 2,846 human and 297 mouse surface/secreted recombinant proteins using recombinant NadA as probe. Efficient NadA binding was revealed on the paired sialic acid-binding immunoglobulin-type lectins receptors 5 and 14 (Siglec-5 and Siglec-14), but not on Siglec-9 therein used as control. The interaction was confirmed by biochemical tools with the determination of the K Competing Interests: V.V., S.U.L., W.P., E.B., K.S., V.M., N.N., and D.M. are employees of GSK. R.R., V.M., K.S., and D.M. report ownership of GSK shares and/or restricted GSK shares. M.M. is an employee of the University of Naples Federico II with a consultancy contract with GSK. |
Databáze: | MEDLINE |
Externí odkaz: |