Identification of potential therapeutic target SPP1 and related RNA regulatory pathway in keloid based on bioinformatics analysis.

Autor:	Xie R; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Yun J; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Li C; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Zhang S; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Zhong A; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Wu J; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Cen Y; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Li Z; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China., Chen J; Department of Burn and Plastic Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
Jazyk:	angličtina
Zdroj:	Annals of medicine [Ann Med] 2024 Dec; Vol. 56 (1), pp. 2382949. Date of Electronic Publication: 2024 Jul 23.
DOI:	10.1080/07853890.2024.2382949
Abstrakt:	Objective: To explore the complex mechanisms of keloid, new approaches have been developed by different strategies. However, conventional treatment did not significantly reduce the recurrence rate. This study aimed to identify new biomarkers and mechanisms for keloid progression through bioinformatics analyses. Methods: In our study, microarray datasets for keloid were downloaded from the GEO database. Differentially expressed genes (DEGs) were identified by R software. Multiple bioinformatics tools were used to identify hub genes, and reverse predict upstream miRNAs and lncRNA molecules of target hub genes. Finally, the total RNA-sequencing technique and miRNA microarray were combined to validate the identified genes. Results: Thirty-one DEGs were screened out and the upregulated hub gene SPP1 was finally identified, which was consistent with our RNA-sequencing analysis results and validation dataset. In addition, a ceRNA network of mRNA (SPP1)-miRNA (miR-181a-5p)-lncRNA (NEAT1, MALAT1, LINC00667, NORAD, XIST and MIR4458HG) was identified by the bioinformatics databases. The results of our miRNA microarray showed that miR-181a-5p was upregulated in keloid, also we found that the lncRNA NEAT1 could affect keloid progression by retrieving the relevant literature. Conclusions: We speculate that SPP1 is a potential candidate biomarker and therapeutic target for patients with keloid, and NEAT1/miR-181a-5p/SPP1 might be the RNA regulatory pathway that regulates keloid formation.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu