Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants.

Autor: Fu H; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Zhong J; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Zhao J; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Huo L; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Wang C; Shanghai Key Laboratory of Plant Molecular Sciences, Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China., Ma D; Shanghai Key Laboratory of Plant Molecular Sciences, Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China., Pan W; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Sun L; Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, 9000, Belgium., Ren Z; Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Fan T; Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai, 200438, China., Wang Z; College of Tropical Crops, Hainan University, Haikou, 570228, China., Wang W; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Lei X; School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China., Yu G; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China., Li J; College of Tropical Crops, Hainan University, Haikou, 570228, China., Zhu Y; Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai, 200438, China., Geelen D; Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, 9000, Belgium., Liu B; College of Life Sciences, South-Central Minzu University, Wuhan, 430074, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Sep; Vol. 243 (6), pp. 2214-2234. Date of Electronic Publication: 2024 Jul 22.
DOI: 10.1111/nph.19978
Abstrakt: Ultraviolet (UV) radiation influences development and genome stability in organisms; however, its impact on meiosis, a special cell division essential for the delivery of genetic information across generations in eukaryotes, has not yet been elucidated. In this study, by performing cytogenetic studies, we reported that UV radiation does not damage meiotic chromosome integrity but attenuates centromere-mediated chromosome stability and induces unreduced gametes in Arabidopsis thaliana. We showed that functional centromere-specific histone 3 (CENH3) is required for obligate crossover formation and plays a role in the protection of sister chromatid cohesion under UV stress. Moreover, we found that UV specifically alters the orientation and organization of spindles and phragmoplasts at meiosis II, resulting in meiotic restitution and unreduced gametes. We determined that UV-induced meiotic restitution does not rely on the UV Resistance Locus8-mediated UV perception and the Tapetal Development and Function1- and Aborted Microspores-dependent tapetum development, but possibly occurs via altered JASON function and downregulated Parallel Spindle1. This study provides evidence that UV radiation influences meiotic genome stability and gametophytic ploidy consistency in flowering plants.
(© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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