| Autor: |
Menéndez E; Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.; Instituto de Investigación en Agrobiotecnología (CIALE), Universidad de Salamanca, Salamanca, Spain.; Grupo de Interacción Planta-Microorganismo, USAL, Unidad Asociada al CSIC por el IRNASA, Salamanca, Spain., Flores-Félix JD; Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.; Instituto de Investigación en Agrobiotecnología (CIALE), Universidad de Salamanca, Salamanca, Spain., Kaur S; Department of Biology, Queen's University, Kingston, Ontario, Canada., diCenzo GC; Department of Biology, Queen's University, Kingston, Ontario, Canada., Young JPW; Department of Biology, University of York, York YO10 5DD, UK., Peix A; Grupo de Interacción Planta-Microorganismo, USAL, Unidad Asociada al CSIC por el IRNASA, Salamanca, Spain.; Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain., Velázquez E; Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.; Instituto de Investigación en Agrobiotecnología (CIALE), Universidad de Salamanca, Salamanca, Spain.; Grupo de Interacción Planta-Microorganismo, USAL, Unidad Asociada al CSIC por el IRNASA, Salamanca, Spain. |
| Abstrakt: |
The species Rhizobium indigoferae and Sinorhizobium kummerowiae were isolated from legume nodules and the 16S rRNA sequences of their respective type strains, CCBAU 71042 T and CCBAU 71714 T , were highly divergent from those of the other species of the genera Rhizobium and Sinorhizobium , respectively. However, the 16S rRNA gene sequences obtained for strains CCBAU 71042 T and CCBAU 71714 T several years after description, were different from the original ones, showing 100 % similarity to the type strains of Rhizobium leguminosarum and Sinorhizobium meliloti , respectively. Phylogenetic analyses of two housekeeping genes, recA and atpD , confirmed the high phylogenetic closeness of strains CCBAU 71042 T and CCBAU 71714 T to the respective type strains of R. leguminosarum and S. meliloti . In the present work, we compared the genomes of the type strains of R. indigoferae and S. kummerowiae available in several culture collections with those of the respective type strains of R. leguminosarum and S. meliloti , some of them obtained in this study. The calculated average nucleotide identity-blast and digital DNA-DNA hybridization values in both cases were higher than those recommended for species differentiation, supporting the proposal for the reclassification of the type strains of R. indigoferae and S. kummerowiae into the species R. leguminosarum and S. meliloti , respectively. |
