Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.
| Autor: | Wong CYY; School of Biological Sciences , The University of Hong Kong, Hong Kong., Tsui HN; School of Biological Sciences , The University of Hong Kong, Hong Kong., Wang Y; School of Biological Sciences , The University of Hong Kong, Hong Kong., Yuen KWY; School of Biological Sciences , The University of Hong Kong, Hong Kong.; School of Biological Sciences , University of Southampton, Southampton SO17 1BJ, UK. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of cell science [J Cell Sci] 2024 Sep 15; Vol. 137 (18). Date of Electronic Publication: 2024 Sep 18. |
| DOI: | 10.1242/jcs.261895 |
| Abstrakt: | Chromosome segregation errors caused by centromere malfunction can lead to chromosome instability and aneuploidy. In Caenorhabditis elegans, the Argonaute protein CSR-1 is essential for proper chromosome segregation, although the specific mechanisms are not fully understood. Here, we investigated how CSR-1 regulates centromere and kinetochore function in C. elegans embryos. We found that depletion of CSR-1 results in defects in mitotic progression and chromosome positioning relative to the spindle pole. Knockdown of CSR-1 does not affect mRNA and protein levels of the centromeric histone H3 variant and CENP-A homolog HCP-3 but does increase the localization of HCP-3 and some kinetochore proteins to the mitotic chromosomes. Such elevation of HCP-3 chromatin localization depends on EGO-1, which is an upstream factor in the CSR-1 RNA interference (RNAi) pathway, and PIWI domain activity of CSR-1. Our results suggest that CSR-1 restricts the level of HCP-3 at the holocentromeres, prevents erroneous kinetochore assembly and thereby promotes accurate chromosome segregation. Our work sheds light on the role of CSR-1 in regulating deposition of HCP-3 on chromatin and centromere function in embryos. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
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