Nanostructured Implant-Tissue Interface Assessment Using a Three-Dimensional Gingival Tissue Equivalent.

Autor:	Llopis-Grimalt MA; Group of Cell Therapy and Tissue Engineering, Department of Fundamental Biology and Health Sciences, Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, Palma 07122, Spain.; Health Research Institute of the Balearic Islands, IdISBa, Palma 07010, Spain., Munar-Bestard M; Group of Cell Therapy and Tissue Engineering, Department of Fundamental Biology and Health Sciences, Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, Palma 07122, Spain.; Health Research Institute of the Balearic Islands, IdISBa, Palma 07010, Spain., Ramis-Munar G; Cellomics Unit, Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, Palma 07122, Spain., Smith D; Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom., Starborg T; Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom., Kadler KE; Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, United Kingdom., Monjo M; Group of Cell Therapy and Tissue Engineering, Department of Fundamental Biology and Health Sciences, Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, Palma 07122, Spain.; Health Research Institute of the Balearic Islands, IdISBa, Palma 07010, Spain., Ramis JM; Group of Cell Therapy and Tissue Engineering, Department of Fundamental Biology and Health Sciences, Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, Palma 07122, Spain.; Health Research Institute of the Balearic Islands, IdISBa, Palma 07010, Spain.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2024 Jul 03; Vol. 9 (28), pp. 30534-30543. Date of Electronic Publication: 2024 Jul 03 (Print Publication: 2024).
DOI:	10.1021/acsomega.4c02253
Abstrakt:	Improved soft tissue integration (STI) around dental implants is key for implant success. The formation of an early and long-lasting transmucosal seal around the implant abutment might help to prevent peri-implantitis, one of the major causes of late implant failure. In natural teeth, collagen fibers are firmly inserted and fixed in the cementum of the tooth and emerge perpendicular to the gingival tissue. In contrast, around dental implants, collagen fibers run predominantly parallel to the implant surface, allowing bacterial migration into the peri-implant interface that might lead to peri-implantitis. Previous studies have shown that nanostructured Ti surfaces improve gingival cell response in monolayer cell cultures. Here, we aimed at evaluating the implant-tissue interface using a 3D gingival tissue equivalent (GTE). First, we evaluated the GTE response to a nanostructured (NN) and machined Ti surface after the stimulation with Porphyromonas gingivalis lipopolysaccharide (LPS), to simulate peri-implantitis conditions. Thus, GTE viability, through MTT assay, the release of metalloproteinase-1 (MMP1) and its inhibitor (TIMP1) through ELISA, and the gene expression of extracellular matrix turnover genes by real-time RT-PCR were analyzed. Second, GTE-implant interaction was characterized by serial block face scanning electron microscopy, and collagen-1 orientation at the tissue-implant interface was analyzed by immunofluorescence. While a similar GTE response to LPS stimulation was found for both implant surfaces, a higher proportion of collagen oriented perpendicular to the implant was observed on the NN implant surface. Thus, our results indicate that the nanostructuration of titanium dental implant abutments could allow the correct orientation of collagen fibers and greater soft tissue sealing, while keeping biocompatibility levels and LPS response comparable. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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