Quercetin alleviates kidney damage caused by mercury Chloride: The protective effects of quercetin on autophagy and inflammation were studied based on TRIM32/TLR4/LC3 pathway.

Autor: Jin F; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Li J; Chengdu Medical College, Chengdu 610500, PR China., Zhao C; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Gu L; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Pu M; Chengdu Medical College, Chengdu 610500, PR China., Jiang S; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Liang M; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Zhao Y; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Shen J; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Agabuwei A; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China., Han Q; School of Public Health, Chengdu Medical College, Chengdu 610500, PR China. Electronic address: hanqin@cmc.edu.cn., Liao D; Chengdu Medical College, Chengdu 610500, PR China; Mianyang Hospital, School of Medicine, University of Electronic Science and Technology of China,Mianyang621000,PR China. Electronic address: liaodanyu@163.com.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Sep; Vol. 248, pp. 108031. Date of Electronic Publication: 2024 Jul 20.
DOI: 10.1016/j.toxicon.2024.108031
Abstrakt: Objective: Mercury is one of the heavy metal pollutants causing serious harm to human health. Quercetin was observed to repair kidney damage through the TLR4/TRIM32 pathway, and the detoxification effect of quercetin on heavy metal poisoning was observed.
Methods: For the study, the researchers divided 40 male mice from the KM strain into five groups: control, HgCl 2 , QU30, HgCl 2 +QU15, and HgCl 2 +QU30. The biological effects of those mice in each group were detected by the biochemical experiment, histopathology experiment and protein expression experiment respectively.
Results: HgCl 2 had effects in increasing the level of malondialdehyde (MDA) and decreasing the activity of antioxidant enzymes (P < 0.05). HgCl 2 induced inflammation by increasing tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and Toll Like Receptor 4 (TLR-4) (P < 0.05). The expression of creatinine (CRE) and urea nitrogen (BUN) showed that HgCl 2 promoted kidney injury. HgCl 2 altered renal tissue integrity and TRIM32 expression which resulted in the increased autophagy associated protein levels of LC3. In contrast, quercetin reduced oxidative stress, autophagy, inflammation and histopathological changes (P < 0.05).
Conclusion: Quercetin has the renal protection effects of anti-inflammation, anti-oxidation and anti-autophagy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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