Biodegradable capsules as a sustainable and accessible container for vitrification of gonadal tissue using the zebrafish animal model.

Autor: de Freitas TR; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: thaiza.rfreitas@gmail.com., Rodrigues RB; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Department of Animal Science and Biological Sciences, Federal University of Santa Maria, Palmeira das Missões, RS, Brazil. Electronic address: rrodrigues1903@gmail.com., Marques LS; Veterinary Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: lismarx@gmail.com., Dantas RV; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: rezinhaa.d@gmail.com., Torres-Lozano KG; Veterinary Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: gelina9611@gmail.com., França TS; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: thalesfranca@gmail.com., Lima LCO; Veterinary Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: larise.carolinevet@gmail.com., Santos FW; Reproduction Biotechnology Laboratory (Biotech), Federal University of Pampa, Uruguaiana, RS, Brazil. Electronic address: francielliweber@yahoo.com.br., Nicoleti ET; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: eduardonicoleti97@hotmail.com., Chaves TF; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: talesfabris2@gmail.com., Streit DP Jr; AQUAM Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Veterinary Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: danilo.streit@ufrgs.br.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2024 Sep; Vol. 116, pp. 104944. Date of Electronic Publication: 2024 Aug 09.
DOI: 10.1016/j.cryobiol.2024.104944
Abstrakt: Cryopreservation of fish gonadal tissue is an important technique for preserving genetic variability. However, this technique involves the use of cryotubes, plastic containers with low degradability that are expensive and difficult to obtain in certain parts of the world. Therefore, this study aimed to evaluate the efficiency of gelatin and hypromellose hard capsules as a sustainable and accessible alternative container to the cryotube for vitrification of zebrafish (Danio rerio) gonadal tissue. The gonadal tissues (testicular or ovarian) were vitrified in cryotubes, hard-gelatin, and hard-hypromellose capsules. Gelatin capsules exhibited comparable efficacy to cryotubes in preserving spermatogonia viability (33.03 ± 10.03 % and 37.96 ± 8.35 %, respectively), whereas hypromellose capsules showed decreased viability (18.38 ± 2.09 %). Immature oocyte viability remained unaffected by the capsule materials, with no difference compared to cryotubes at all oocyte stages (Primary Growth: p < 0.0001; Cortical Alveolar: p < 0.0001; Vitellogenic: p < 0.0001). Mitochondrial activity and lipid peroxidation demonstrated no difference among cryotubes and capsules for both gonadal tissues. However, antioxidant activity was notably higher in gelatin capsules (Testes: 147.2 ± 32.32 μg; Ovary: 87.98 ± 10.91 μg) than in cryotubes (Testes: 81.04 ± 26.05 μg; Ovary: 54.35 ± 11.23 μg) and hypromellose capsules (Testes: 62.36 ± 17.10 μg; Ovary: 63.96 ± 7.51 μg), likely due to the inherent antioxidant properties of gelatin. The results obtained in this study demonstrate that the cryotube can be replaced by gelatin capsules for vitrification of both gonadal tissues of zebrafish, being a sustainable and accessible alternative as it is a low-cost and environmentally friendly container.
Competing Interests: Declaration of competing interest TSF and DPSJ filed (September/2022) a patent (BR10202201781) application on the methodology described in the study. The remaining authors have no conflicts of interest that could be perceived as prejudicing the impartiality of the research reported.
(Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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