Identification of substituted variants of plastoquinone-9 as potent and specific photosynthetic inhibitors in cyanobacteria and plants.

Autor: Latimer S; Department of Horticultural Sciences, University of Florida, Gainesville, FL, 32611, USA. Electronic address: scottlatimer@ufl.edu., Stutts LR; Department of Horticultural Sciences, University of Florida, Gainesville, FL, 32611, USA. Electronic address: laurenrstutts@ufl.edu., Basset GJ; Department of Horticultural Sciences, University of Florida, Gainesville, FL, 32611, USA. Electronic address: gbasset@ufl.edu.
Jazyk: angličtina
Zdroj: Phytochemistry [Phytochemistry] 2024 Oct; Vol. 226, pp. 114225. Date of Electronic Publication: 2024 Jul 19.
DOI: 10.1016/j.phytochem.2024.114225
Abstrakt: The unprenylated benzoquinones 2,3,5,6-tetramethyl-1,4-benzoquinone (duroquinone), 2-chloro-1,4-benzoquinone (CBQ), 2,6-dimethyl-1,4-benzoquinone (DMBQ), 2,6-dichloro-1,4-benzoquinone (DCBQ), and 2,6-dimethoxy-1,4-benzoquinone (DMOBQ) were tested as putative antimetabolites of plastoquinone-9, a vital electron and proton carrier of oxygenic phototrophs. Duroquinone and CBQ were the most effective at inhibiting the growth of the cyanobacterium Synechocystis sp. PCC 6803 either in photomixotrophic or photoautotrophic conditions. Duroquinone, a close structural analog of the photosynthetic inhibitor methyl-plastoquinone-9, was found to possess genuine bactericidal activity towards Synechocystis at a concentration as low as 10 μM, while at the same concentration CBQ acted only as a mild bacteriostat. In contrast, only duroquinone displayed marked cytotoxicity in axenically-grown Arabidopsis, resulting in damages to photosystem II and hindered net CO 2 assimilation. Metabolite profiling targeted to photosynthetic cofactors and pigments indicated that in Arabidopsis duroquinone does not directly inhibit plastoquinone-9 biosynthesis. Taken together, these data indicate that duroquinone offers prospects as an algicide and herbicide.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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