Tumor micro-environment induced TRAIL secretion from engineered macrophages for anti-tumor therapy.

Autor: Zhu Q; Applied Biology Laboratory, College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China., Huang X; Rocrock Biotech Co., Ltd, Suzhou 215000, China; Department of General Practice Medicine, Shengjing Hospital of China Medical University, Shenyang 110022, China., Deng B; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Guan L; Applied Biology Laboratory, College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China., Zhou H; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Shi B; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Liu J; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Shan X; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Fang X; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Xu F; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Li H; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Liu X; Rocrock Biotech Co., Ltd, Suzhou 215000, China., Yin X; Applied Biology Laboratory, College of Environmental and Safety Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China; Rocrock Biotech Co., Ltd, Suzhou 215000, China. Electronic address: xiushanyin@me.com., Zhang L; Research Center of Bioengineering, the Medical Innovation Research Division of Chinese PLA General Hospital, Beijing 100853, China. Electronic address: marbleluo@126.com.
Jazyk: angličtina
Zdroj: Cellular immunology [Cell Immunol] 2024 Sep-Oct; Vol. 403-404, pp. 104857. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1016/j.cellimm.2024.104857
Abstrakt: The high plasticity and long-term persistency make macrophages excellent vehicles for delivering anti-tumor cytokines. Macrophage delivery of chemokines and cytokines shows potential in tumor therapy. TRAIL, a promising anti-tumor cytokine, induces apoptosis in tumor cells with low toxicity to normal cells. However, its off-target toxicity and limited stability have limited its clinical progress. Here, we engineered macrophages with Mono-TRAIL and Tri-TRAIL and found that Tri-TRAIL had higher cytotoxic activity against tumor cells than Mono-TRAIL in vitro. To target the tumor microenvironment (TME), we generated macrophages secreting trimeric TRAIL (Tri-TRAIL-iM) induced by the TME-specific promoter Arg1. The Tri-TRAIL-iM cells displayed high specific activatable activity in cell-based co-culture assay and tumor-baring mice models. In addition, we demonstrated that compared to macrophages over-expressing TRAIL under a non-inducible promoter, Tri-TRAIL-iM could more effectively induce apoptosis in cancer cells, inhibit tumor growth, and reduce systemic side effects. This strategy of inducing TRAIL delivery holds great potential for cancer therapy. It is promising to be combined with other engineering methods to maximize the therapeutic effects of solid tumors.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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