Alpha-Glucosidase Inhibitory Effects of Flavonoids, Phenolic Acids and Iridoids Isolated From Vinca Soneri: In Vitro and In Silico Perspectives.

Autor: Uyanır E; Faculty of Pharmacy, Department of Pharmacognosy, Hacettepe University, TR-06100, Ankara, Türkiye., Šoral M; Institute of Chemistry, Analytical Department, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovak Republic., Seyhan G; Faculty of Pharmacy, Department of Biochemistry, Karadeniz Technical University, TR-61080, Trabzon, Türkiye., Akkaya D; Faculty of Pharmacy, Department of Biochemistry, Karadeniz Technical University, TR-61080, Trabzon, Türkiye., Barut B; Faculty of Pharmacy, Department of Biochemistry, Karadeniz Technical University, TR-61080, Trabzon, Türkiye., Sari S; Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Hacettepe University, TR-06100, Ankara, Türkiye., Duman H; Faculty of Science, Department of Biology, Gazi University, TR-06500, Ankara, Türkiye., Renda G; Faculty of Pharmacy, Department of Pharmacognosy, Karadeniz Technical University, 61080, Trabzon, Türkiye., Şöhretoğlu D; Faculty of Pharmacy, Department of Pharmacognosy, Hacettepe University, TR-06100, Ankara, Türkiye.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Oct; Vol. 21 (10), pp. e202401386. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1002/cbdv.202401386
Abstrakt: Various Vinca species have been traditionally used for their antihypertensive, sedative, and hemostatic properties, as well as for treating diabetes. In this study, some flavonoids, phenolic acids and iridoids were isolated from an endemic Vinca species, Vinca soneri for the first time. α-Glucosidase inhibitory effects of the isolates were tested and kaempferol-3-O-α-rhamnopyranosyl (1→6) β-galactopyranoside (1) was found to be the most active one with an IC 50 value of 285.73 ±7.35 μM. Enzyme kinetic assay revealed that it inhibited α-glucosidase in competitive manner. Molecular geometry of 1 was predicted and Frontier molecular orbital analysis was performed using Density Functional Theory (DFT) calculations. Molecular docking and MM-GBSA calculations predicted good fit for 1 in the enzyme active site and key interactions with the catalytic residues. As a result, current study identifies 1 as a promising competitive α-glucosidase inhibitor to be developed as a potential antidiabetic drug candidate.
(© 2024 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
Externí odkaz: