Exploring the role of mesenchymal stem cells in modulating immune responses via Treg and Th2 cell activation: insights from mouse model of multiple sclerosis.

Autor: Sadeghnejad A; Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran., Pazoki A; Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran., Yazdanpanah E; Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Esmaeili SA; Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Yousefi B; Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran., Sadighi-Moghaddam B; Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran., Baharlou R; Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran., Haghmorad D; Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.; Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Jazyk: angličtina
Zdroj: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2024 Nov; Vol. 132 (11), pp. 888-899. Date of Electronic Publication: 2024 Jul 19.
DOI: 10.1111/apm.13456
Abstrakt: Multiple sclerosis is a demyelinating neurodegenerative disease, and its animal model, experimental autoimmune encephalomyelitis (EAE), exhibits immunological and clinical similarities. The study aimed to examine mechanisms underlying therapeutic effects of mesenchymal stem cell administration in EAE. C57BL/6 mice were separated into control and treatment groups (T1, T2, and T3); EAE was induced in all animals. Clinical examinations were conducted daily, and on 25th day, animals were sacrificed, and spinal cord was stained for histological analysis. Additionally, spleen cell proliferation assay, assessments of cytokine, and gene expression in both spinal cord and spleen cells were performed. The results indicated a significant reduction in clinical symptoms among treatment groups compared to control group. Histological analyses revealed decreased infiltration of lymphocytes into the spinal cord and reduced demyelinated areas in treatment groups compared to control group. Cytokine production of IL-10, TGF-β, and IL-4 were significantly enhanced and IFN-γ and TNF-α in treatment groups were decreased relative to control group. Also, gene expression of CTLA-4, PD-1, IL-27, and IL-33 indicated a significant increase in treatment groups. The administration of MSCs significantly improved clinical symptoms, attenuated inflammation, and reduced spinal cord demyelination in EAE, suggesting a potential protective effect on disease progression.
(© 2024 Scandinavian Societies for Pathology, Medical Microbiology and Immunology.)
Databáze: MEDLINE
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