The potential role of collagen type VII in breast cancer proliferation.

Autor: Pérez-Díaz S; Department of Medical and Translational Biology, Umeå University, Umeå, SE-901 87, Sweden. sergio.perez.diaz@ki.se.; Department of Laboratory Medicine, Division of Clinical Physiology, Karolinska Institute, Stockholm, Sweden. sergio.perez.diaz@ki.se., Lindberg J; Department of Medical and Translational Biology, Umeå University, Umeå, SE-901 87, Sweden.; Department of Diagnostics and Intervention, Plastic Surgery and Surgery, Umeå University, Umeå, Sweden., Anerillas LO; Department of Medical and Translational Biology, Umeå University, Umeå, SE-901 87, Sweden., Kingham PJ; Department of Medical and Translational Biology, Umeå University, Umeå, SE-901 87, Sweden., Sund M; Department of Diagnostics and Intervention, Plastic Surgery and Surgery, Umeå University, Umeå, Sweden.; Department of Surgery/CLINICUM, University of Helsinki and Helsinki University Hospital, Helsinki, Finland., Rask G; Department of Diagnostics and Intervention, Plastic Surgery and Surgery, Umeå University, Umeå, Sweden., Svensson J; Department of Statistics, Umeå School of Business, Economics and Statistics, Umeå University, Umeå, Sweden., Jansson M; Department of Diagnostics and Intervention, Plastic Surgery and Surgery, Umeå University, Umeå, Sweden., Wiberg R; Department of Medical and Translational Biology, Umeå University, Umeå, SE-901 87, Sweden.; Department of Diagnostics and Intervention, Plastic Surgery and Surgery, Umeå University, Umeå, Sweden.
Jazyk: angličtina
Zdroj: Cancer cell international [Cancer Cell Int] 2024 Jul 20; Vol. 24 (1), pp. 254. Date of Electronic Publication: 2024 Jul 20.
DOI: 10.1186/s12935-024-03449-4
Abstrakt: Background: Breast cancer is the most common cancer in women. Cancer cells can persist in a prolonged dormant state for years without any clinical evidence of disease creating an urgent need to better understand the molecular mechanisms leading to relapse. This study aimed to identify extracellular matrix (ECM) components associated with hypoxia-induced breast cancer dormancy. The effects of selected ECM proteins on breast cancer cell proliferation were analyzed, along with their correlation with established prognostic markers in human breast cancer tissue.
Materials and Methods: Screening of extracellular matrix proteins was performed in hypoxia-induced dormant MCF-7 breast cancer cells. Proliferation of MCF-7 cells in vitro was subsequently determined in the presence of recombinant ColVII. Adipose tissue-derived mesenchymal stem cells (AdMSCs) subpopulation overexpressing ColVII were indirectly isolated by ColVII receptor integrin-α6 specific antibodies. AdMSCs- MCF-7 3D spheroid cultures were generated to model solid tumour conditions. In addition, the association between ColVII and various prognostic markers was evaluated in clinical samples of human breast cancer tissue.
Results: Dormant MCF-7 cells showed an elevated expression of ColVII while MCF-7 cells cultured on ColVII exhibited reduced proliferation in vitro. In AdMSCs-MCF-7 3D spheroids, a reduced proliferation of MCF-7 cells was observed in Int-α6 + / ColVII high compared with Int-α6 -/ ColVII low AdMSCs spheroids. In human tissue, high ColVII expression correlated to several positive prognostic markers. Staining for Cytokeratin-5 revealed that ColVII high -expressing cells were predominantly myoepithelial cells.
Conclusion: ColVII is associated with reduced proliferation of breast cancer cells in vitro. ColVII is strongly expressed in myoepithelial cells and in breast cancer tissue the high ColVII expression correlates with several well-known positive prognostic markers, highlighting its potential as a prognostic marker in breast cancer.
(© 2024. The Author(s).)
Databáze: MEDLINE
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